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Biomolecules 2014, 4(2), 402-418;

Design of Catalytically Amplified Sensors for Small Molecules

Department of Chemistry, Syracuse University, Syracuse, NY 13244, USA
Authors to whom correspondence should be addressed.
Received: 6 February 2014 / Revised: 21 March 2014 / Accepted: 26 March 2014 / Published: 17 April 2014
(This article belongs to the Special Issue Metal Binding Proteins)
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Catalytically amplified sensors link an allosteric analyte binding site with a reactive site to catalytically convert substrate into colored or fluorescent product that can be easily measured. Such an arrangement greatly improves a sensor’s detection limit as illustrated by successful application of ELISA-based approaches. The ability to engineer synthetic catalytic sites into non-enzymatic proteins expands the repertoire of analytes as well as readout reactions. Here we review recent examples of small molecule sensors based on allosterically controlled enzymes and organometallic catalysts. The focus of this paper is on biocompatible, switchable enzymes regulated by small molecules to track analytes both in vivo and in the environment. View Full-Text
Keywords: sensor; protein design; signal amplification; catalysis sensor; protein design; signal amplification; catalysis

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Makhlynets, O.V.; Korendovych, I.V. Design of Catalytically Amplified Sensors for Small Molecules. Biomolecules 2014, 4, 402-418.

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