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Biomolecules 2013, 3(2), 334-350;

Angling for Uniqueness in Enzymatic Preparation of Glycosides

Institute of Biomolecular Chemistry, National Research Council, Via Campi Flegrei, 34, Pozzuoli 80078, Naples, Italy
Received: 4 May 2013 / Revised: 23 May 2013 / Accepted: 3 June 2013 / Published: 13 June 2013
(This article belongs to the Special Issue Enzymes and Their Biotechnological Applications)
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In the early days of biocatalysis, limitations of an enzyme modeled the enzymatic applications; nowadays the enzyme can be engineered to be suitable for the process requirements. This is a general bird’s-eye view and as such cannot be specific for articulated situations found in different classes of enzymes or for selected enzymatic processes. As far as the enzymatic preparation of glycosides is concerned, recent scientific literature is awash with examples of uniqueness related to the features of the biocatalyst (yield, substrate specificity, regioselectivity, and resistance to a particular reaction condition). The invention of glycosynthases is just one of the aspects that has thrust forward the research in this field. Protein engineering, metagenomics and reaction engineering have led to the discovery of an expanding number of novel enzymes and to the setting up of new bio-based processes for the preparation of glycosides. In this review, new examples from the last decade are compiled with attention both to cases in which naturally present, as well as genetically inserted, characteristics of the catalysts make them attractive for biocatalysis. View Full-Text
Keywords: biocatalysis; glycosides; enzymes; glycosidases; glycosynthases biocatalysis; glycosides; enzymes; glycosidases; glycosynthases

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Trincone, A. Angling for Uniqueness in Enzymatic Preparation of Glycosides. Biomolecules 2013, 3, 334-350.

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