Bioimprinting as a Receptor for Detection of Kwakhurin
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Reagents
2.2. Bioimprinting Procedure
2.3. Preparation of Kwa-HSA and Kwa-HRP Conjugates
2.4. icELISA Using biOVA
2.5. Indirect ELISA (iELISA) Using biOVA
2.6. cELBIA Using biOVA
2.7. Non-Competitive ELBIA (ncELBIA) Using biOVA
3. Results
3.1. Development of the icELISA and cELBIA Using biOVA
3.1.1. Optimization of Various Parameters for icELISA
3.1.2. Optimization of Various Parameters for cELBIA
3.1.3. Characterization of biOVA
3.1.4. Determination of Kwa by the icELISA and cELBIA Using biOVA
3.2. Investigation of Candidate Proteins for the Bioimprinting of Kwa
4. Discussion
4.1. Development of the icELISA and cELBIA Using biOVA for Quantitative Analysis of Kwa
4.2. Investigation of Candidate Proteins for the Bioimprinting of Kwa
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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CRs (%) | |||
---|---|---|---|
Class | Compound | biOVA | MAb 11F a |
Isoflavonoids | Kwa | 100 | 100 |
Daidzein | 23.4 | <0.005 | |
Genistein | 25.5 | <0.005 | |
Isoflavonoid glycosides | Daidzin | 9.4 | <0.005 |
Genistin | 5.2 | <0.005 | |
Puerarin | <0.1 | <0.005 |
Sample Name | icELISA Using biOVA | cELBIA Using biOVA | icELISA Using MAb 11F | |||
---|---|---|---|---|---|---|
Kwa Amount (% wt./dry wt.) | CV (%) | Kwa Amount (% wt./dry wt.) | CV (%) | Kwa Amount (% wt./dry wt.) | CV (%) | |
P. candollei root without bark | 4.23 × 10−4 | 11.8 | 4.49 × 10−4 | 9.0 | 1.94 × 10−4 | 7.0 |
P. candollei root bark 1 | 1.56 × 10−2 | 12.1 | 1.43 × 10−2 | 7.2 | 1.09 × 10−2 | 8.2 |
P. candollei root bark 2 | 1.22 × 10−3 | 6.2 | 1.11 × 10−3 | 9.3 | 1.29 × 10−3 | 0.4 |
Supplement 1 | 9.19 × 10−5 | 4.3 | ND | ND | 9.94 × 10−6 | 10.3 |
Supplement 2 | 6.89 × 10−3 | 11.7 | 9.65 × 10−3 | 4.9 | 6.23 × 10−3 | 4.5 |
Supplement 3 | 2.04 × 10−3 | 8.1 | 2.14 × 10−3 | 9.3 | 1.27 × 10−3 | 5.0 |
Supplement 4 | 3.36 × 10−4 | 8.7 | 4.12 × 10−4 | 6.5 | 2.38 × 10−4 | 9.0 |
Supplement 5 | ND | ND | ND | ND | 5.73 × 10−6 | 9.4 |
CRs (%) | |||
---|---|---|---|
Class | Compound | biMAb 2H2 | biMAb 1D2 |
Isoflavonoids | Kwa | 100 | 100 |
Daidzein | <0.1 | 0.2 | |
Genistein | <0.1 | 7.1 | |
Isoflavonoid glycosides | Daidzin | <0.1 | 5.4 |
Genistin | 0.1 | 19.6 | |
Puerarin | 7.0 | 57.3 |
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Sakamoto, S.; Minami, K.; Nuntawong, P.; Yusakul, G.; Putalun, W.; Tanaka, H.; Fujii, S.; Morimoto, S. Bioimprinting as a Receptor for Detection of Kwakhurin. Biomolecules 2022, 12, 1064. https://doi.org/10.3390/biom12081064
Sakamoto S, Minami K, Nuntawong P, Yusakul G, Putalun W, Tanaka H, Fujii S, Morimoto S. Bioimprinting as a Receptor for Detection of Kwakhurin. Biomolecules. 2022; 12(8):1064. https://doi.org/10.3390/biom12081064
Chicago/Turabian StyleSakamoto, Seiichi, Kei Minami, Poomraphie Nuntawong, Gorawit Yusakul, Waraporn Putalun, Hiroyuki Tanaka, Shunsuke Fujii, and Satoshi Morimoto. 2022. "Bioimprinting as a Receptor for Detection of Kwakhurin" Biomolecules 12, no. 8: 1064. https://doi.org/10.3390/biom12081064