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Open AccessArticle

Extra Virgin Olive Oil Phenols Vasodilate Rat Mesenteric Resistance Artery via Phospholipase C (PLC)-Calcium Microdomains-Potassium Channels (BKCa) Signals

1
Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, Italy
2
Maternal and Fetal Health Research Centre, Division of Developmental Biology and Medicine, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9WL, UK
3
Manchester Academic Health Science Centre, Manchester University NHS Foundation Trust, St. Mary’s Hospital, Manchester M13 9WL, UK
4
Department of Nutrition Sciences, University of Alabama at Birmingham, Birmingham, AL 35294, USA
5
Department of Obstetrics, Gynecology and Reproductive Science, University of Vermont, Burlington, VT 05405, USA
*
Author to whom correspondence should be addressed.
Biomolecules 2021, 11(2), 137; https://doi.org/10.3390/biom11020137
Received: 31 December 2020 / Revised: 12 January 2021 / Accepted: 18 January 2021 / Published: 21 January 2021
Recent evidence suggests that the reason Extra Virgin Olive Oil (EVOO) lowers blood pressure and reduces the risk of developing hypertension is partly due to minor components of EVOO, such as phenols. However, little is still known about the mechanism(s) through which EVOO phenols mediate anti-hypertensive effects. The aim of the present study was to investigate the mechanisms of action of EVOO phenols on mesenteric resistance arteries. A pressure myograph was used to test the effect of EVOO phenols on isolated mesenteric arteries in the presence of specific inhibitors of: (1) BKca channels (Paxillin, 10−5 M); (2) L-type calcium channels (Verapamil, 10−5 M); (3) Ryanodine receptor, RyR (Ryanodine, 10−5 M); (4) inositol 1,4,5-triphosphate receptor, IP3R, (2-Aminoethyl diphenylborinate, 2-APB, 3 × 10−3 M); (5) phospholipase C, PLC, (U73122, 10−5 M), and (6) GPCR-Gαi signaling, (Pertussis Toxin, 10−5 M). EVOO phenols induced vasodilation of mesenteric arteries in a dose-dependent manner, and this effect was reduced by pre-incubation with Paxillin, Verapamil, Ryanodine, 2-APB, U73122, and Pertussis Toxin. Our data suggest that EVOO phenol-mediated vasodilation requires activation of BKca channels potentially through a local increase of subcellular calcium microdomains, a pivotal mechanism on the base of artery vasodilation. These findings provide novel mechanistic insights for understanding the vasodilatory properties of EVOO phenols on resistance arteries. View Full-Text
Keywords: mesenteric artery; BKCa channels; Ca2+ microdomains; PLC; GPCR-Gαi mesenteric artery; BKCa channels; Ca2+ microdomains; PLC; GPCR-Gαi
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MDPI and ACS Style

D’Agostino, R.; Barberio, L.; Gatto, M.; Tropea, T.; De Luca, M.; Mandalà, M. Extra Virgin Olive Oil Phenols Vasodilate Rat Mesenteric Resistance Artery via Phospholipase C (PLC)-Calcium Microdomains-Potassium Channels (BKCa) Signals. Biomolecules 2021, 11, 137. https://doi.org/10.3390/biom11020137

AMA Style

D’Agostino R, Barberio L, Gatto M, Tropea T, De Luca M, Mandalà M. Extra Virgin Olive Oil Phenols Vasodilate Rat Mesenteric Resistance Artery via Phospholipase C (PLC)-Calcium Microdomains-Potassium Channels (BKCa) Signals. Biomolecules. 2021; 11(2):137. https://doi.org/10.3390/biom11020137

Chicago/Turabian Style

D’Agostino, Rossana; Barberio, Laura; Gatto, Mariacarmela; Tropea, Teresa; De Luca, Maria; Mandalà, Maurizio. 2021. "Extra Virgin Olive Oil Phenols Vasodilate Rat Mesenteric Resistance Artery via Phospholipase C (PLC)-Calcium Microdomains-Potassium Channels (BKCa) Signals" Biomolecules 11, no. 2: 137. https://doi.org/10.3390/biom11020137

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