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The Role of the C-Terminal Lysine of S100P in S100P-Induced Cell Migration and Metastasis

Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Biosciences Building, Crown Street, Liverpool L69 7ZB, UK
College of Health and Life Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Wiesława Leśniak and Anna Filipek
Biomolecules 2021, 11(10), 1471;
Received: 12 September 2021 / Revised: 29 September 2021 / Accepted: 2 October 2021 / Published: 6 October 2021
S100P protein is a potent inducer of metastasis in a model system, and its presence in cancer cells of patients is strongly associated with their reduced survival times. A well-established Furth Wistar rat metastasis model system, methods for measuring cell migration, and specific inhibitors were used to study pathways of motility-driven metastasis. Cells expressing C-terminal mutant S100P proteins display markedly-reduced S100P-driven metastasis in vivo and cell migration in vitro. These cells fail to display the low focal adhesion numbers observed in cells expressing wild-type S100P, and the mutant S100P proteins exhibit reduced biochemical interaction with non-muscle myosin heavy chain isoform IIA in vitro. Extracellular inhibitors of the S100P-dependent plasminogen activation pathway reduce, but only in part, wild-type S100P-dependent cell migration; they are without effect on S100P-negative cells or cells expressing C-terminal mutant S100P proteins and have no effect on the numbers of focal adhesions. Recombinant wild-type S100P protein, added extracellularly to S100P-negative cells, stimulates cell migration, which is abolished by these inhibitors. The results identify at least two S100P-dependent pathways of migration, one cell surface and the other intracellularly-linked, and identify its C-terminal lysine as a target for inhibiting multiple migration-promoting activities of S100P protein and S100P-driven metastasis. View Full-Text
Keywords: S100P; membrane; metastasis; cell migration; myosin llA S100P; membrane; metastasis; cell migration; myosin llA
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MDPI and ACS Style

Ismail, T.M.; Gross, S.R.; Lancaster, T.; Rudland, P.S.; Barraclough, R. The Role of the C-Terminal Lysine of S100P in S100P-Induced Cell Migration and Metastasis. Biomolecules 2021, 11, 1471.

AMA Style

Ismail TM, Gross SR, Lancaster T, Rudland PS, Barraclough R. The Role of the C-Terminal Lysine of S100P in S100P-Induced Cell Migration and Metastasis. Biomolecules. 2021; 11(10):1471.

Chicago/Turabian Style

Ismail, Thamir M., Stephane R. Gross, Tara Lancaster, Philip S. Rudland, and Roger Barraclough. 2021. "The Role of the C-Terminal Lysine of S100P in S100P-Induced Cell Migration and Metastasis" Biomolecules 11, no. 10: 1471.

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