Novel Broccoli Sulforaphane-Based Analogues Inhibit the Progression of Pancreatic Cancer without Side Effects
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemical Syntheses of Sulforaphane Analogues
2.2. Human Tumor Cell Lines
2.3. Reagents
2.4. Cell Viability Assay
2.5. Colony Forming Assay
2.6. NCI-60 Human Tumor Cell Lines Screen
2.7. Tumor Xenotransplantation on Fertilized Chicken Eggs
2.8. Immunohistochemical Staining
2.9. Caenorhabditis Elegans Survival
2.10. miRNA Microarray Profiling
2.11. miRNA In Silico Analysis
2.12. Statistical Analysis
3. Results
3.1. Chemical Synthesis of Sulforaphane Derivatives
3.2. SF102 and SF134 Are Cytotoxic in All the Several Evaluated Tumor Cells
3.3. SF102 and SF134 Inhibit Tumor Growth without Obvious Side Effects
3.4. Sulforaphane, SF102 and SF134 Induce Differential microRNA Expression and NF-κB Signaling
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Georgikou, C.; Buglioni, L.; Bremerich, M.; Roubicek, N.; Yin, L.; Gross, W.; Sticht, C.; Bolm, C.; Herr, I. Novel Broccoli Sulforaphane-Based Analogues Inhibit the Progression of Pancreatic Cancer without Side Effects. Biomolecules 2020, 10, 769. https://doi.org/10.3390/biom10050769
Georgikou C, Buglioni L, Bremerich M, Roubicek N, Yin L, Gross W, Sticht C, Bolm C, Herr I. Novel Broccoli Sulforaphane-Based Analogues Inhibit the Progression of Pancreatic Cancer without Side Effects. Biomolecules. 2020; 10(5):769. https://doi.org/10.3390/biom10050769
Chicago/Turabian StyleGeorgikou, Christina, Laura Buglioni, Maximilian Bremerich, Nico Roubicek, Libo Yin, Wolfgang Gross, Carsten Sticht, Carsten Bolm, and Ingrid Herr. 2020. "Novel Broccoli Sulforaphane-Based Analogues Inhibit the Progression of Pancreatic Cancer without Side Effects" Biomolecules 10, no. 5: 769. https://doi.org/10.3390/biom10050769