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Metabolites 2012, 2(1), 195-213;

Shotgun Lipidomics by Sequential Precursor Ion Fragmentation on a Hybrid Quadrupole Time-of-Flight Mass Spectrometer

AB SCIEX, 71 Four Valley Dr. Concord, ON L4K4V8, Canada
Zora Biosciences, Biologinkuja 1, Espoo, FI-2150, Finland
Author to whom correspondence should be addressed.
Received: 24 December 2011 / Revised: 10 February 2012 / Accepted: 13 February 2012 / Published: 20 February 2012
(This article belongs to the Special Issue Lipidomics)
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Shotgun lipidomics has evolved into a myriad of multi-dimensional strategies for molecular lipid characterization, including bioinformatics tools for mass spectrum interpretation and quantitative measurements to study systems-lipidomics in complex biological extracts. Taking advantage of spectral mass accuracy, scan speed and sensitivity of improved quadrupole linked time-of-flight mass analyzers, we developed a bias-free global lipid profiling acquisition technique of sequential precursor ion fragmentation called MS/MSALL. This generic information-independent tandem mass spectrometry (MS) technique consists of a Q1 stepped mass isolation window through a set mass range in small increments, fragmenting and recording all product ions and neutral losses. Through the accurate MS and MS/MS information, the molecular lipid species are resolved, including distinction of isobaric and isomeric species, and composed into more precise lipidomic outputs. The method demonstrates good reproducibility and at least 3 orders of dynamic quantification range for isomeric ceramides in human plasma. More than 400 molecular lipids in human plasma were uncovered and quantified in less than 12 min, including acquisitions in both positive and negative polarity modes. We anticipate that the performance of sequential precursor ion fragmentation both in quality and throughput will lead to the uncovering of new avenues throughout the biomedical research community, enhance biomarker discovery and provide novel information target discovery programs as it will prospectively shed new insight into affected metabolic and signaling pathways. View Full-Text
Keywords: MS/MSALL; lipidomics; lipid; QTOF; mass spectrometry MS/MSALL; lipidomics; lipid; QTOF; mass spectrometry

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Simons, B.; Kauhanen, D.; Sylvänne, T.; Tarasov, K.; Duchoslav, E.; Ekroos, K. Shotgun Lipidomics by Sequential Precursor Ion Fragmentation on a Hybrid Quadrupole Time-of-Flight Mass Spectrometer. Metabolites 2012, 2, 195-213.

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