Green Synthesis of Silver Nanoparticles Using Bellevalia Flexuosa Leaves Extract
Abstract
1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of B. flexuosa Methanolic Extract
2.3. Green Synthesis of Silver Nanoparticles (AgNPs)
2.4. Characterization of the Green-Synthesized AgNPs
2.5. Antibacterial Susceptibility Test
3. Results and Discussion
3.1. Characterization of the AgNPs
3.2. Antimicrobial Activity of the AgNPs
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
- Mody, V.; Siwale, R.; Singh, A.; Mody, H. Introduction to metallic nanoparticles. J. Pharm. Bioallied Sci. 2010, 2, 282. [Google Scholar] [CrossRef] [PubMed]
- Singla, R.; Guliani, A.; Kumari, A.; Yadav, S.K. Metallic nanoparticles, toxicity issues and applications in medicine. In Nanoscale Materials in Targeted Drug Delivery, Theragnosis and Tissue Regeneration; Springer: Singapore, 2016; pp. 41–80. [Google Scholar] [CrossRef]
- Ahmad, M.Z.; Akhter, S.; Jain, G.K.; Rahman, M.; Pathan, S.A.; Ahmad, F.J.; Khar, R.K. Metallic nanoparticles: Technology overview & drug delivery applications in oncology. Expert Opin. Drug Deliv. 2010, 7, 927–942. [Google Scholar] [CrossRef] [PubMed]
- Kumar, H.; Venkatesh, N.; Bhowmik, H.; Kuila, A. Metallic Nanoparticle: A Review. Biomed. J. Sci. Tech. Res. 2018, 4, 3765–3775. [Google Scholar] [CrossRef]
- Mandava, K. Biological and Non-biological Synthesis of Metallic Nanoparticles: Scope for Current Pharmaceutical Research. Indian J. Pharm. Sci. 2017, 79, 501–512. [Google Scholar] [CrossRef]
- Mohanty, S.; Mishra, S.; Jena, P.; Jacob, B.; Sarkar, B.; Sonawane, A. An investigation on the antibacterial, cytotoxic, and antibiofilm efficacy of starch-stabilized silver nanoparticles. Nanomed. Nanotechnol. Biol. Med. 2012, 8, 916–924. [Google Scholar] [CrossRef]
- Shaikh, S.; Nazam, N.; Rizvi, S.M.D.; Ahmad, K.; Baig, M.H.; Lee, E.J.; Choi, I. Mechanistic Insights into the Antimicrobial Actions of Metallic Nanoparticles and Their Implications for Multidrug Resistance. Int. J. Mol. Sci. 2019, 20, 2468. [Google Scholar] [CrossRef]
- Kumar, V.S.; Nagaraja, B.M.; Shashikala, V.; Padmasri, A.H.; Madhavendra, S.S.; Raju, B.D.; Rao, K.S.R. Highly efficient Ag/C catalyst prepared by electro-chemical deposition method in controlling microorganisms in water. J. Mol. Catal. A Chem. 2004, 223, 313–319. [Google Scholar] [CrossRef]
- Ahmed, S.; Ahmad, M.; Swami, B.L.; Ikram, S. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise. J. Adv. Res. 2016, 7, 17–28. [Google Scholar] [CrossRef]
- Echegoyen, Y.; Nerín, C. Nanoparticle release from nano-silver antimicrobial food containers. Food Chem. Toxicol. 2013, 62, 16–22. [Google Scholar] [CrossRef]
- Rai, M.; Yadav, A.; Gade, A. Silver nanoparticles as a new generation of antimicrobials. Biotechnol. Adv. 2009, 27, 76–83. [Google Scholar] [CrossRef]
- Teirumnieks, E.; Balchev, I.; Ghalot, R.S.; Lazov, L. Antibacterial and anti-viral effects of silver nanoparticles in medicine against COVID-19—A review. Laser Phys. 2021, 31, 013001. [Google Scholar] [CrossRef]
- Sun, Y.; Yin, Y.; Mayers, B.T.; Herricks, T.; Xia, Y. Uniform silver nanowires synthesis by reducing AgNO3 with ethylene glycol in the presence of seeds and poly(vinyl pyrrolidone). Chem. Mater. 2002, 14, 4736–4745. [Google Scholar] [CrossRef]
- Callegari, A.; Tonti, D.; Chergui, M. Photochemically Grown Silver Nanoparticles with Wavelength-Controlled Size and Shape. Nano Lett. 2003, 3, 1565–1568. [Google Scholar] [CrossRef]
- Dimitrijevic, N.M.; Bartels, D.M.; Jonah, C.D.; Takahashi, K.; Rajh, T. Radiolytically induced formation and optical absorption spectra of colloidal silver nanoparticles in supercritical ethane. J. Phys. Chem. B 2001, 105, 954–959. [Google Scholar] [CrossRef]
- Swami, A.; Selvakannan, P.R.; Pasricha, R.; Sastry, M. One-step synthesis of ordered two-dimensional assemblies of silver nanoparticles by the spontaneous reduction of silver ions by pentadecylphenol langmuir monolayers. J. Phys. Chem. B 2004, 108, 19269–19275. [Google Scholar] [CrossRef]
- Joseph, S.; Mathew, B. Microwave assisted facile green synthesis of silver and gold nanocatalysts using the leaf extract of Aerva lanata. Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 2015, 136, 1371–1379. [Google Scholar] [CrossRef]
- Abid, J.P.; Wark, A.W.; Brevet, P.F.; Girault, H.H. Preparation of silver nanoparticles in solution from a silver salt by laser irradiation. Chem. Commun. 2002, 7, 792–793. [Google Scholar] [CrossRef]
- Naik, R.R.; Stringer, S.J.; Agarwal, G.; Jones, S.E.; Stone, M.O. Biomimetic synthesis and patterning of silver nanoparticles. Nat. Mater. 2002, 1, 169–172. [Google Scholar] [CrossRef]
- Ahmad, A.; Mukherjee, P.; Senapati, S.; Mandal, D.; Khan, M.I.; Kumar, R.; Sastry, M. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids Surfaces B Biointerfaces 2003, 28, 313–318. [Google Scholar] [CrossRef]
- Shah, M.; Fawcett, D.; Sharma, S.; Tripathy, S.K.; Poinern, G.E.J. Green Synthesis of Metallic Nanoparticles via Biological Entities. Materials 2015, 8, 7278–7308. [Google Scholar] [CrossRef]
- Gardea-Torresdey, J.L.; Gomez, E.; Peralta-Videa, J.R.; Parsons, J.G.; Troiani, H.; Jose-Yacaman, M. Alfalfa Sprouts: A Natural Source for the Synthesis of Silver Nanoparticles. Langmuir 2003, 19, 1357–1361. [Google Scholar] [CrossRef]
- Salem, S.S.; Fouda, A. Green Synthesis of Metallic Nanoparticles and Their Prospective Biotechnological Applications: An Overview. Biol. Trace Elem. Res. 2021, 199, 344–370. [Google Scholar] [CrossRef] [PubMed]
- Rahim, M.; Rizvi, S.M.D.; Iram, S.; Khan, S.; Bagga, P.S.; Khan, M.S. Interaction of green nanoparticles with cells and organs. In Inorganic Frameworks as Smart Nanomedicines; William Andrew Publishing: Norwich, NY, USA, 2018; pp. 185–237. [Google Scholar] [CrossRef]
- Mariselvam, R.; Ranjitsingh, A.J.A.; Usha Raja Nanthini, A.; Kalirajan, K.; Padmalatha, C.; Mosae Selvakumar, P. Green synthesis of silver nanoparticles from the extract of the inflorescence of Cocos nucifera (Family: Arecaceae) for enhanced antibacterial activity. Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 2014, 129, 537–541. [Google Scholar] [CrossRef]
- Krishnaraj, C.; Jagan, E.G.; Rajasekar, S.; Selvakumar, P.; Kalaichelvan, P.T.; Mohan, N. Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surf. B Biointerfaces 2010, 76, 50–56. [Google Scholar] [CrossRef] [PubMed]
- Taifour, H. Jordan Plant Red List. In Proceedings of the Royal Botanic Garden 1st Annual Scientific Day, Amman, Jordan, 12 January 2012; Royal Botanic Garden 1st Annual Scientific Day: Amman, Jordan, 2012. [Google Scholar]
- El-Elimat, T.; Rivera-Chávez, J.; Burdette, J.E.; Czarnecki, A.; Alhawarri, M.B.; Al-Gharaibeh, M.; Alali, F.; Oberlies, N.H. Cytotoxic homoisoflavonoids from the bulbs of Bellevalia flexuosa. Fitoterapia 2018, 127, 201–206. [Google Scholar] [CrossRef]
- Lin, L.-G.; Liu, Q.-Y.; Ye, Y. Naturally Occurring Homoisoflavonoids and Their Pharmacological Activities. Planta Med. 2014, 80, 1053–1066. [Google Scholar] [CrossRef]
- Rashidipour, M.; Heydari, R. Biosynthesis of silver nanoparticles using extract of olive leaf: Synthesis and in vitro cytotoxic effect on MCF-7 cells. J. Nanostructure Chem. 2014, 4, 112. [Google Scholar] [CrossRef]
- Chang, Y.C.; Yang, C.Y.; Sun, R.L.; Cheng, Y.F.; Kao, W.C.; Yang, P.C. Rapid single cell detection of Staphylococcus aureus by aptamer-conjugated gold nanoparticles. Sci. Rep. 2013, 3, 1863. [Google Scholar] [CrossRef]
- Luna-Sánchez, J.L.; Jiménez-Pérez, J.L.; Carbajal-Valdez, R.; Lopez-Gamboa, G.; Pérez-González, M.; Correa-Pacheco, Z.N. Green synthesis of silver nanoparticles using Jalapeño Chili extract and thermal lens study of acrylic resin nanocomposites. Thermochim. Acta 2019, 678, 178314. [Google Scholar] [CrossRef]
- Salehi, S.; Sadat Shandiz, S.A.; Ghanbar, F.; Darvish, M.R.; Ardestani, M.S.; Mirzaie, A.; Jafari, M. Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties. Int. J. Nanomed. 2016, 11, 1835–1846. [Google Scholar] [CrossRef]
- Anigol, L.B.; Balekundri, S.G.; Charantimath, J.S.; Gurubasavaraj, P.M. Effect of Concentration and pH on the Size of Silver Nanoparticles Synthesized by Green Chemistry. Org. Med. Chem. 2017, 3, 124–128. [Google Scholar] [CrossRef]
- Dehnavi, A.S.; Raisi, A.; Aroujalian, A. Control Size and Stability of Colloidal Silver Nanoparticles with Antibacterial Activity Prepared by a Green Synthesis Method. Synth. React. Inorg. Met. Nano-Metal Chem. 2013, 43, 543–551. [Google Scholar] [CrossRef]
- Chutrakulwong, F.; Thamaphat, K.; Limsuwan, P. Photo-irradiation induced green synthesis of highly stable silver nanoparticles using durian rind biomass: Effects of light intensity, exposure time and pH on silver nanoparticles formation. J. Phys. Commun. 2020, 4, 095015. [Google Scholar] [CrossRef]
- Dadashpour, M.; Firouzi-Amandi, A.; Pourhassan-Moghaddam, M.; Maleki, M.J.; Soozangar, N.; Jeddi, F.; Nouri, M.; Zarghami, N.; Pilehvar-Soltanahmadi, Y. Biomimetic synthesis of silver nanoparticles using Matricaria chamomilla extract and their potential anticancer activity against human lung cancer cells. Mater. Sci. Eng. C. Mater. Biol. Appl. 2018, 92, 902–912. [Google Scholar] [CrossRef] [PubMed]
- Razavi, R.; Amiri, M.; Alshamsi, H.A.; Eslaminejad, T.; Salavati-Niasari, M. Green synthesis of Ag nanoparticles in oil-in-water nano-emulsion and evaluation of their antibacterial and cytotoxic properties as well as molecular docking. Arab. J. Chem. 2021, 14, 103323. [Google Scholar] [CrossRef]
- Sánchez, G.R.; Castilla, C.L.; Gómez, N.B.; García, A.; Marcos, R.; Carmona, E.R. Leaf extract from the endemic plant Peumus boldus as an effective bioproduct for the green synthesis of silver nanoparticles. Mater. Lett. 2016, 183, 255–260. [Google Scholar] [CrossRef]
- Elzey, S.; Grassian, V.H. Agglomeration, isolation and dissolution of commercially manufactured silver nanoparticles in aqueous environments. J. Nanoparticle Res. 2010, 12, 1945–1958. [Google Scholar] [CrossRef]
- Kumar, P.; Govindaraju, M.; Senthamilselvi, S.; Premkumar, K. Photocatalytic degradation of methyl orange dye using silver (Ag) nanoparticles synthesized from Ulva lactuca. Colloids Surf. B. Biointerfaces 2013, 103, 658–661. [Google Scholar] [CrossRef] [PubMed]
- Wang, M.; Li, H.; Li, Y.; Mo, F.; Li, Z.; Chai, R.; Wang, H. Dispersibility and Size Control of Silver Nanoparticles with Anti-Algal Potential Based on Coupling Effects of Polyvinylpyrrolidone and Sodium Tripolyphosphate. Nanomater 2020, 10, 1042. [Google Scholar] [CrossRef]
- Danaei, M.; Dehghankhold, M.; Ataei, S.; Hasanzadeh Davarani, F.; Javanmard, R.; Dokhani, A.; Khorasani, S.; Mozafari, M.R. Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems. Pharmaceutics 2018, 10, 57. [Google Scholar] [CrossRef] [PubMed]
- Demeler, B.; Nguyen, T.L.; Gorbet, G.E.; Schirf, V.; Brookes, E.H.; Mulvaney, P.; El-Ballouli, A.O.; Pan, J.; Bakr, O.M.; Demeler, A.K.; et al. Characterization of size, anisotropy, and density heterogeneity of nanoparticles by sedimentation velocity. Anal. Chem. 2014, 86, 7688–7695. [Google Scholar] [CrossRef]
- Chorny, M.; Fishbein, I.; Danenberg, H.D.; Golomb, G. Lipophilic drug loaded nanospheres prepared by nanoprecipitation: Effect of formulation variables on size, drug recovery and release kinetics. J. Control. Release 2002, 83, 389–400. [Google Scholar] [CrossRef]
- Cakić, M.; Glišić, S.; Cvetković, D.; Cvetinov, M.; Stanojević, L.; Danilović, B.; Cakić, K. Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Produced fromFumaria officinalis L. Plant Extract. Colloid J. 2018, 80, 803–813. [Google Scholar] [CrossRef]
- Marshall, A.T.; Haverkamp, R.G.; Davies, C.E.; Parsons, J.G.; Gardea-Torresdey, J.L.; van Agterveld, D. Accumulation of Gold Nanoparticles in Brassic Juncea. Int. J. Phytoremediation 2007, 9, 197–206. [Google Scholar] [CrossRef]
- Ahmad, N.; Sharma, S. Green Synthesis of Silver Nanoparticles Using Extracts of Ananas comosus. Green Sustain. Chem. 2012, 2, 141–147. [Google Scholar] [CrossRef]
- Christy, A.J.; Umadevi, M. Synthesis and characterization of monodispersed silver nanoparticles. Adv. Nat. Sci. Nanosci. Nanotechnol. 2012, 3, 035013. [Google Scholar] [CrossRef]
- Khatami, M.; Sharifi, I.; Nobre, M.A.L.; Zafarnia, N.; Aflatoonian, M.R. Waste-grass-mediated green synthesis of silver nanoparticles and evaluation of their anticancer, antifungal and antibacterial activity. Green Chem. Lett. Rev. 2018, 11, 125–134. [Google Scholar] [CrossRef]
- Rajasekar, P.; Palanisamy, S.; Anjali, R.; Vinosha, M.; Thillaieswari, M.; Malaikozhundan, B.; Boomi, P.; Saravanan, M.; You, S.G.; Prabhu, N.M. Cladophora fascicularis Mediated Silver Nanoparticles: Assessment of Their Antibacterial Activity Against Aeromonas hydrophila. J. Clust. Sci. 2020, 31, 673–683. [Google Scholar] [CrossRef]
- Lateef, A.; Azeez, M.A.; Asafa, T.B.; Yekeen, T.A.; Akinboro, A.; Oladipo, I.C.; Azeez, L.; Ajibade, S.E.; Ojo, S.A.; Gueguim-Kana, E.B.; et al. Biogenic synthesis of silver nanoparticles using a pod extract of Cola nitida: Antibacterial and antioxidant activities and application as a paint additive. J. Taibah Univ. Sci. 2016, 10, 551–562. [Google Scholar] [CrossRef]
- Rutherford, D.; Jíra, J.; Kolářová, K.; Matolínová, I.; Mičová, J.; Remeš, Z.; Rezek, B. Growth Inhibition of Gram-Positive and Gram-Negative Bacteria by Zinc Oxide Hedgehog Particles. Int. J. Nanomed. 2021, 16, 3541. [Google Scholar] [CrossRef] [PubMed]
- Alshareef, A.; Laird, K.; Cross, R.B.M. Shape-dependent antibacterial activity of silver nanoparticles on Escherichia coli and Enterococcus faecium bacterium. Appl. Surf. Sci. 2017, 424, 310–315. [Google Scholar] [CrossRef]
- Rai, M.K.; Deshmukh, S.D.; Ingle, A.P.; Gade, A.K. Silver nanoparticles: The powerful nanoweapon against multidrug-resistant bacteria. J. Appl. Microbiol. 2012, 112, 841–852. [Google Scholar] [CrossRef]
- Murugesan, A.K.; Pannerselvam, B.; Javee, A.; Rajenderan, M.; Thiyagarajan, D. Facile green synthesis and characterization of Gloriosa superba L. tuber extract-capped silver nanoparticles (GST-AgNPs) and its potential antibacterial and anticancer activities against A549 human cancer cells. Environ. Nanotechnol. Monit. Manag. 2021, 15, 100460. [Google Scholar] [CrossRef]
- Morones, J.R.; Elechiguerra, J.L.; Camacho, A.; Holt, K.; Kouri, J.B.; Ramírez, J.T.; Yacaman, M.J. The bactericidal effect of silver nanoparticles. Nanotechnology 2005, 16, 2346–2353. [Google Scholar] [CrossRef] [PubMed]
- Guzman, M.; Dille, J.; Godet, S. Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteria. Nanomedicine 2012, 8, 37–45. [Google Scholar] [CrossRef] [PubMed]
Property | AgNO3 Concentration | ||
---|---|---|---|
1 mM | 5 mM | 10 mM | |
Hydrodynamic diameter (nm) | 86.5 ± 1.8 | 352.9 ± 795.2 | 1219.0 ± 339.3 |
PdI | 0.45 ± 0.0 | 0.93 ± 0.0 | 0.81 ± 0.1 |
ZP (mV) | −27 ± 0.3 | −36 ± 3.6 | −6 ± 3.5 |
No. | IR Value | Functional Group Detection | Remark |
---|---|---|---|
1 | 719 cm−1 | –CH | –CH aromatic bending |
2 | 902 cm−1 | –OH | –OH bending of carboxylic |
3 | 1078, 1170 cm−1 | C–O or –C–O–C– | C–O stretching |
4 | 1380 cm−1 | –CH | C–H bending of alkane |
5 | 1471 cm−1 | –CH | C–H bending of alkane |
6 | 1543, 1575 cm−1 | –C=C– | –C=C– stretching vibration |
7 | 1743 cm−1 | C=O | C=O stretching of carbonyl |
8 | 2850, 2920 cm−1 | –CH | –CH stretching of alkyl |
9 | 3373 cm−1 | –OH | –OH stretching of alcohol/phenol |
Bacterial Strain | Negative Control | AgNPs |
---|---|---|
P. aeruginosa (0.174) | 1.665 ± 0.029 | 1.491 ± 0.033 |
E. coli (0.272) | 2.040 ± 0.031 | 1.768 ± 0.027 |
K. pneumonia (0.26) | 2.012 ± 0.001 | 1.752 ± 0.030 |
S. enterica (0.303) | 1.897 ± 0.047 | 1.594 ± 0.014 |
E. faecalis (0.7) | 1.879 ± 0.0502 | 1.113 ± 0.055 |
S. epidermidis (0.33) | 1.917 ± 0.011 | 1.587 ± 0.012 |
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Al-Nemrawi, N.; Hameedat, F.; El-Elimat, T. Green Synthesis of Silver Nanoparticles Using Bellevalia Flexuosa Leaves Extract. Sci. Pharm. 2022, 90, 60. https://doi.org/10.3390/scipharm90040060
Al-Nemrawi N, Hameedat F, El-Elimat T. Green Synthesis of Silver Nanoparticles Using Bellevalia Flexuosa Leaves Extract. Scientia Pharmaceutica. 2022; 90(4):60. https://doi.org/10.3390/scipharm90040060
Chicago/Turabian StyleAl-Nemrawi, Nusaiba, Fatima Hameedat, and Tamam El-Elimat. 2022. "Green Synthesis of Silver Nanoparticles Using Bellevalia Flexuosa Leaves Extract" Scientia Pharmaceutica 90, no. 4: 60. https://doi.org/10.3390/scipharm90040060
APA StyleAl-Nemrawi, N., Hameedat, F., & El-Elimat, T. (2022). Green Synthesis of Silver Nanoparticles Using Bellevalia Flexuosa Leaves Extract. Scientia Pharmaceutica, 90(4), 60. https://doi.org/10.3390/scipharm90040060