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LC-HRMS-Based Profiling: Antibacterial and Lipase Inhibitory Activities of Some Medicinal Plants for the Remedy of Obesity

Biological Chemistry Laboratory, Central Department of Chemistry, Tribhuvan University, Kirtipur 44618, Nepal
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Academic Editor: Roman B. Lesyk
Sci. Pharm. 2022, 90(3), 55; https://doi.org/10.3390/scipharm90030055
Received: 14 August 2022 / Revised: 3 September 2022 / Accepted: 5 September 2022 / Published: 8 September 2022
Globally, obesity is a serious health concern that causes numerous diseases, including type 2 diabetes, hypertension, cardiovascular diseases, etc. Medicinal plants have been used to aid in weight loss since ancient times. Thus, this research is focused on the exploration of pancreatic lipase inhibitory activity and secondary metabolite profiling of Bergenia ciliata, Mimosa pudica, and Phyllanthus emblica, selected based on an ethnobotanical survey. The lipase inhibition was investigated using 4-nitrophenyl butyrate (p-NPB) as a substrate. To uncover further therapeutic potentials of these medicinal plants, antimicrobial activity and minimum inhibitory concentration (MIC) of the extracts were also determined. The ethyl acetate plant extracts showed higher antimicrobial activity against Staphylococcus aureus, Escherichia coli, Salmonella typhi, and Shigella sonnei. The MIC of ethyl acetate extracts of medicinal plants considered in this study ranges from 1.56 to 6.25 mg/mL. The hexane fraction of Mimosa pudica and Phyllanthus emblica showed a higher lipase inhibitory activity as compared to others, with IC50 values of 0.49 ± 0.02 and 2.45 ± 0.003 mg/mL, respectively. In the case of Bergenia ciliata, the methanolic extract inhibited lipase more effectively than others, with an IC50 value of 1.55 ± 0.02 mg/mL (IC50 value of orlistat was 179.70 ± 3.60 µg/mL). A mass spectrometry analysis of various solvent/solvent partition fractions (extracts) revealed 29 major secondary metabolites. The research offers a multitude of evidence for using medicinal plants as antiobesity and antimicrobial agents. View Full-Text
Keywords: medicinal plants; lipase; antibacterial activity; mass spectrometry medicinal plants; lipase; antibacterial activity; mass spectrometry
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MDPI and ACS Style

Sapkota, B.K.; Khadayat, K.; Aryal, B.; Bashyal, J.; Jaisi, S.; Parajuli, N. LC-HRMS-Based Profiling: Antibacterial and Lipase Inhibitory Activities of Some Medicinal Plants for the Remedy of Obesity. Sci. Pharm. 2022, 90, 55. https://doi.org/10.3390/scipharm90030055

AMA Style

Sapkota BK, Khadayat K, Aryal B, Bashyal J, Jaisi S, Parajuli N. LC-HRMS-Based Profiling: Antibacterial and Lipase Inhibitory Activities of Some Medicinal Plants for the Remedy of Obesity. Scientia Pharmaceutica. 2022; 90(3):55. https://doi.org/10.3390/scipharm90030055

Chicago/Turabian Style

Sapkota, Basanta Kumar, Karan Khadayat, Babita Aryal, Jyoti Bashyal, Shankar Jaisi, and Niranjan Parajuli. 2022. "LC-HRMS-Based Profiling: Antibacterial and Lipase Inhibitory Activities of Some Medicinal Plants for the Remedy of Obesity" Scientia Pharmaceutica 90, no. 3: 55. https://doi.org/10.3390/scipharm90030055

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