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Article

Development of HPLC Method for Simultaneous Determination of Ibuprofen and Chlorpheniramine Maleate

1
College of Pharmacy, Ninevah University, Mosul 41002, Iraq
2
College of Pharmacy, University of Mosul, Mosul 41002, Iraq
3
Department of Chemical and Biochemical Engineering, Rutgers University, Piscataway, NJ 08854, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Susi Burgalassi
Sci. Pharm. 2022, 90(3), 53; https://doi.org/10.3390/scipharm90030053
Received: 8 July 2022 / Revised: 6 August 2022 / Accepted: 10 August 2022 / Published: 30 August 2022
One of the most prevalent over-the-counter cold and cough medications is the chlorpheniramine maleate (CPM)–ibuprofen (IBF) combination. A reversed-phase high-performance liquid chromatography (RP-HPLC) method was effectively optimized and developed for the simultaneous detection of chlorpheniramine maleate and ibuprofen in a pharmaceutical formulation. The mobile phase for the RP-HPLC method was an isocratic combination of acetonitrile and 0.01 M acetate buffer at pH 3.8 (55:45; v/v) on an Eclipse Plus C18 reversed phase column. An ultraviolet (UV) detector with a wavelength of 225 nm was used to detect the analytes at a flow rate of 1.0 mL/min. CPM and IBF were satisfactorily eluted, with mean retention times of 2.09 and 6.27 min, respectively. The approach was shown to be linear (R2 > 0.9998 for CPM and 0.9992 for IBF), precise (% RSD 3.02% for CPM and 3.48% for IBF), accurate (% recoveries 97.7–98.9% for CPM and 101–104.5% for IBF), specific, easy to use, sensitive, quick, and robust. Limits of detection (LODs) were found to be 10 and 27 μg/mL for CPM and IBF, respectively. Without interference from excipients, the validated method could be utilized in regular quality control analysis of various dosage combinations of hard gelatin capsules containing CPM and IBF. View Full-Text
Keywords: RP-HPLC; ibuprofen; chlorpheniramine maleate; pharmaceutical formulation RP-HPLC; ibuprofen; chlorpheniramine maleate; pharmaceutical formulation
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MDPI and ACS Style

Aldewachi, H.; Omar, T.A. Development of HPLC Method for Simultaneous Determination of Ibuprofen and Chlorpheniramine Maleate. Sci. Pharm. 2022, 90, 53. https://doi.org/10.3390/scipharm90030053

AMA Style

Aldewachi H, Omar TA. Development of HPLC Method for Simultaneous Determination of Ibuprofen and Chlorpheniramine Maleate. Scientia Pharmaceutica. 2022; 90(3):53. https://doi.org/10.3390/scipharm90030053

Chicago/Turabian Style

Aldewachi, Hasan, and Thamer A. Omar. 2022. "Development of HPLC Method for Simultaneous Determination of Ibuprofen and Chlorpheniramine Maleate" Scientia Pharmaceutica 90, no. 3: 53. https://doi.org/10.3390/scipharm90030053

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