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Caesalpinia sappan L. Ameliorates Scopolamine-Induced Memory Deficits in Mice via the cAMP/PKA/CREB/BDNF Pathway

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Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
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Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
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Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
*
Author to whom correspondence should be addressed.
Academic Editor: Thierry Besson
Sci. Pharm. 2021, 89(2), 29; https://doi.org/10.3390/scipharm89020029
Received: 22 April 2021 / Revised: 31 May 2021 / Accepted: 8 June 2021 / Published: 18 June 2021
Memory is an essential aspect of human cognition. A decrease in this aspect is well associated with Alzheimer’s disease (AD). The development of a novel cognitive enhancer (CE) may help overcome AD-related problems. In this study, we evaluated the CE effect of Caesalpinia sappan L. (CS) in memory deficit mice. Administration of its ethanolic extract (250 and 500 mg/kg body weight (BW)) and brazilin (5 and 10 mg/kg BW) ameliorated the scopolamine-amnesic effect, as evidenced by significant decreases (p < 0.01, p < 0.05) in the escape latency time and increases (p < 0.01) in the percentage of time spent in the target quadrant of the Morris water maze test. We also examined the cyclic adenosine monophosphate (cAMP) level, protein kinase A (PKA) activity, and protein expression levels of phosphorylated cAMP response element binding (pCREB) and brain-derived neurotrophic factor (BDNF) in hippocampal tissues to elucidate the underlying molecular mechanism. Results showed that CS wood ethanolic extract and brazilin not only significantly increase (p < 0.01, p < 0.05) cAMP levels and PKA activity but also significantly enhance (p < 0.01, p < 0.05) the expression level of pCREB and BDNF in the hippocampus. These findings indicate that CS activates the cAMP/PKA/CREB/BDNF pathway. Taken together, our results demonstrate that CS is a promising herb that could be developed as a CE agent. View Full-Text
Keywords: brazilin; Caesalpinia sappan L.; cognitive enhancer; cognitive impairment; learning and memory; signaling pathway brazilin; Caesalpinia sappan L.; cognitive enhancer; cognitive impairment; learning and memory; signaling pathway
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MDPI and ACS Style

Helmi, H.; Fakhrudin, N.; Nurrochmad, A.; Ikawati, Z. Caesalpinia sappan L. Ameliorates Scopolamine-Induced Memory Deficits in Mice via the cAMP/PKA/CREB/BDNF Pathway. Sci. Pharm. 2021, 89, 29. https://doi.org/10.3390/scipharm89020029

AMA Style

Helmi H, Fakhrudin N, Nurrochmad A, Ikawati Z. Caesalpinia sappan L. Ameliorates Scopolamine-Induced Memory Deficits in Mice via the cAMP/PKA/CREB/BDNF Pathway. Scientia Pharmaceutica. 2021; 89(2):29. https://doi.org/10.3390/scipharm89020029

Chicago/Turabian Style

Helmi, Helmi, Nanang Fakhrudin, Arief Nurrochmad, and Zullies Ikawati. 2021. "Caesalpinia sappan L. Ameliorates Scopolamine-Induced Memory Deficits in Mice via the cAMP/PKA/CREB/BDNF Pathway" Scientia Pharmaceutica 89, no. 2: 29. https://doi.org/10.3390/scipharm89020029

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