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Article

Quercetin Inhibits Colorectal Cancer Cells Induced-Angiogenesis in Both Colorectal Cancer Cell and Endothelial Cell through Downregulation of VEGF-A/VEGFR2

1
Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
2
Faculty of Medicine Ramathibodi Hospital, Chakri Naruebodindra Medical Institute, Mahidol University, Samut Prakan 10540, Thailand
*
Author to whom correspondence should be addressed.
Academic Editor: Thierry Besson
Sci. Pharm. 2021, 89(2), 23; https://doi.org/10.3390/scipharm89020023
Received: 31 March 2021 / Revised: 10 May 2021 / Accepted: 21 May 2021 / Published: 25 May 2021
Colorectal cancer (CRC) aggressiveness is caused by cancer angiogenesis which promotes the cancer growth and metastasis associated with poor prognosis and poor survival. The vascular endothelial growth factor-A (VEGF-A) and its receptor (VEGFR-2) form the major signaling pathway in cancer angiogenesis. This study aimed to investigate the anti-angiogenesis activity of quercetin in both colorectal cancer cells and endothelial cells. The tube formation of human vein endothelial cells (HUVECs) was determined by using conditioned media of HT-29 cells treated with quercetin co-cultured with HUVECs. The VEGF-A and NF-κB p65 protein expressions in the quercetin-treated HT-29 cells were determined by fluorescence assay and Western blot analysis. The VEGFR-2 protein expression in HUVECs was determined after they were co-cultured with the quercetin-treated HT-29 cells. Quercetin markedly decreased the HT-29 cell-induced angiogenesis in HUVECs. NF-κB p65 and VEGF-A protein expression were also inhibited by quercetin. Moreover, quercetin significantly inhibited VEGFR-2 expression and translocation in HUVECs after they were co-cultured with high dose quercetin-treated HT-29 cells. Taken together, quercetin had an anti-angiogenesis effect on VEGF-A inhibition related to the NF-κB signaling pathway in the HT-29 cells and reduced VEGFR-2 expression and translocation in HUVECs. View Full-Text
Keywords: colorectal cancer; angiogenesis; quercetin; NF-κB; VEGF-A; VEGFR-2 colorectal cancer; angiogenesis; quercetin; NF-κB; VEGF-A; VEGFR-2
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MDPI and ACS Style

Uttarawichien, T.; Kamnerdnond, C.; Inwisai, T.; Suwannalert, P.; Sibmooh, N.; Payuhakrit, W. Quercetin Inhibits Colorectal Cancer Cells Induced-Angiogenesis in Both Colorectal Cancer Cell and Endothelial Cell through Downregulation of VEGF-A/VEGFR2. Sci. Pharm. 2021, 89, 23. https://doi.org/10.3390/scipharm89020023

AMA Style

Uttarawichien T, Kamnerdnond C, Inwisai T, Suwannalert P, Sibmooh N, Payuhakrit W. Quercetin Inhibits Colorectal Cancer Cells Induced-Angiogenesis in Both Colorectal Cancer Cell and Endothelial Cell through Downregulation of VEGF-A/VEGFR2. Scientia Pharmaceutica. 2021; 89(2):23. https://doi.org/10.3390/scipharm89020023

Chicago/Turabian Style

Uttarawichien, Tamonwan; Kamnerdnond, Chantra; Inwisai, Tasanee; Suwannalert, Prasit; Sibmooh, Nathawut; Payuhakrit, Witchuda. 2021. "Quercetin Inhibits Colorectal Cancer Cells Induced-Angiogenesis in Both Colorectal Cancer Cell and Endothelial Cell through Downregulation of VEGF-A/VEGFR2" Sci. Pharm. 89, no. 2: 23. https://doi.org/10.3390/scipharm89020023

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