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Voltammetric Behaviour of Drug Molecules as a Predictor of Metabolic Liabilities

School of Biosciences, University of Birmingham, Edgbaston B15 2TT, UK
Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK
School of Pharmacy, University of Birmingham, Edgbaston B15 2TT, UK
Author to whom correspondence should be addressed.
Sci. Pharm. 2020, 88(4), 46;
Submission received: 15 September 2020 / Revised: 30 September 2020 / Accepted: 3 October 2020 / Published: 13 October 2020


Electron transfer plays a vital role in drug metabolism and underlying toxicity mechanisms. Currently, pharmaceutical research relies on pharmacokinetics (PK) and absorption, distribution, metabolism, elimination and toxicity (ADMET) measurements to understand and predict drug reactions in the body. Metabolic stability (and toxicity) prediction in the early phases of the drug discovery and development process is key in identifying a suitable lead compound for optimisation. Voltammetric methods have the potential to overcome the significant barrier of new drug failure rates, by giving insight into phase I metabolism events which can have a direct bearing on the stability and toxicity of the parent drug being dosed. Herein, we report for the first time a data-mining investigation into the voltammetric behaviour of reported drug molecules and their correlation with metabolic stability (indirectly measured via t½), as a potential predictor of drug stability/toxicity in vivo. We observed an inverse relationship between oxidation potential and drug stability. Furthermore, we selected and prepared short- (<10 min) and longer-circulation (>2 h) drug molecules to prospectively survey the relationship between oxidation potential and stability.

Graphical Abstract

1. Introduction

Electron transfer is an innate property of biological processes in the body [1]. When xenobiotics are introduced to the body (e.g., drug molecules) they encounter a variety of potential redox modulations, including cytochrome p450 (CYP450) enzymes. This metabolic redox biotransformation of the drug facilitates the elimination of the xenobiotic as a more polar drug metabolite [2]. However, redox biotransformations are not always a detoxification process [3]. Oxidation is the primary mechanism of reactive electrophilic metabolite formation, and these in turn react with nucleophiles such as DNA or proteins by forming irreversible covalent bonds, which leads to adverse reactions [4]. These redox events can be measured by voltammetric techniques [5].
One barrier to bringing a new drug to market is the high failure rate. Current methods rely on pharmacokinetics and ADMET measurements [2] in order to identify lead compounds and their optimisation [6]. Electrochemistry (EC) is a relatively new and innovative approach to supporting drug discovery [3]. EC is an alternative method to study drug metabolism and toxicities in early phases by mimicking phase I metabolism [7,8,9,10]. Crucially, most marketed drugs (including pro-drugs) show electroactivity at solid electrodes [4,11].
Our interest in connecting the voltammetric behaviour of drug molecules to their biological properties is derived from our research into the electrochemistry of small molecules [12,13,14] and adverse drug reactions [15,16]. Recent disparate research has found connections between the redox behaviour of a drug and its biological potency [17,18,19] and the oxidation potential and toxicity of MDMA (Ecstasy) derivatives [20,21] and thiobenzamides [22], and voltammetric studies for assessing electrogenerated drug metabolisms [23] as well as a study of xeniobiotics in the environment [24].
Inspired by these connections between the biological properties and voltammetric behaviours of selected drug molecules, we report on our investigations into the emerging connectivity between oxidation potential and drug stability, via:
  • Data mining the existing literature for the half-life and oxidation potential values of known drugs;
  • Correlating standardised redox measurements with stability measures;
  • Analysing examples of short and longer circulating drug molecules to determine their voltammetric parameters.
We hypothesised that oxidation potential correlates with drug stability, as it is assumed lower oxidation potential (OP) indicates an easier biotransformation. Thus, a drug compound with a shorter half-life (t1/2) may have a lower OP.

2. Materials and Methods

2.1. Data Mining

A systematic approach to locating relevant literature sources was adopted using the search engines and databases Sci-Finder, Reaxys and PubChem. Key operator descriptors and Boolean logic were used {oxidation potential; voltammetry; drug; metabolism; half-life; redox} to generate the list of drugs (up until July 2020) that had both a reported redox value and a surrogate biological measure of metabolic stability, although not necessarily from the same source.
Reference electrode data was standardised (where provided) using

2.2. General Points

All commercial reagents and solvents were used as received without further purification. All novel voltammetry experiments were performed using an Autolab potentiostat (PGSTAT 100N, The Netherlands). The progress of reactions was monitored by thin layer chromatography (TLC) using Merck silica gel 60 F254 plates, which were visualized with UV light. Reactions were purified using flash column chromatography (ethyl acetate: petroleum ether) using high-purity grade, pore size 60 Å, 200- 400 mesh particle size silica gel (Sigma-Aldrich, Gillingham, UK).
1H and 13C-NMR spectra were recorded on a JEOL ECS 400 MHz NMR spectrometer. 1H and 13C NMR chemical shifts (δ) are reported in parts per million relative to tetramethylsilane (TMS), with the solvent resonance employed as the internal standard (CDCl3 at 7.26 ppm, CDCl3 at 77.2 ppm). Data are reported as follows: chemical shift, multiplicity (s = singlet, br s = broad singlet, d = doublet, t = triplet, q = quartet, qt = quintet, sext = sextet, m = multiplet and combinations thereof), coupling constants (Hz) and integration. Low- and high-resolution mass spectrometry analysis was obtained using an Agilent 6450 LC-MS/MS system in electrospray ionisation positive mode (ESI+).

2.3. General Procedures for Linear Sweep Voltammetry (LSV)

LSV experiments were performed using a glassy carbon electrode (GCE, geometric area = 0.071 cm2) as the working electrode (WE), a platinum wire was used as a counter electrode (CE) and Ag/AgCl wire was used as a reference electrode (RE). Tetrabutylammonium perchlorate (TBAP) was used as the supporting electrolyte in a MeCN:MeOH (10:1) solvent mixture. Prior to each experiment and in-between scan rate studies, the GCE was polished manually, first with 1.0 μm diamond spray (Kemet, Maidstone, UK) on a smooth velvet polishing pad (BASi, West Lafayette, IN, USA). The GCE was rinsed with distilled water and a second polishing was performed using 0.25 μm diamond spray (Kemet) on a smooth velvet polishing pad (BASi, West Lafayette, IN, USA). Finally, the GCE was rinsed with distilled water and then dried prior to the experiment. The linear regression equations were calculated by the least squares method using Microsoft Excel® software.

2.4. Compound Characterisation

2.4.1. 3-Methoxy-4-hydroxyphenylacetic Acid Propyl Ester (2)

In a pressure tube equipped with magnetic stirrer, 4-hydroxy-3-methoxyphenethyl alcohol, 1, (12.0 mmol) was dissolved in anhydrous propan-1-ol (0.27 M, 21.0 mL). To this solution, concentrated sulfuric acid (1.0 mL) was added and the solution was heated at 100 °C for 5 h. Upon completion, the solvent was removed under reduced pressure. The resulting oil was diluted with ethyl acetate (40.0 mL), and washed with saturated sodium bicarbonate (20.0 mL), distilled water (20.0 mL) and brine (20.0 mL). The organic layer was dried (MgSO4), filtered and concentrated in vacuo to afford the title compound as a red oil (2.45 g, 86%) (Figures S1 and S2). 1H-NMR (400 MHz, CDCl3) δ: ppm 6.90–6.80 (m, 1H), 6.81 (d, J = 1.4 Hz, 1H), 6.80–6.70 (m, 1H), 5.60 (s, 1H), 4.05 (t, J = 6.6 Hz, 2H), 3.88 (s, 3H), 3.54 (s, 2H), 1.71–1.52 (m, 2H), 0.91 (t, J = 7.3 Hz, 3H); 13C-NMR (101 MHz, CDCl3) δ: ppm 172.5, 146.9, 145.0, 125.8, 122.0, 114.8, 112.2, 66.5, 55.6, 40.9, 21.9, 10.3; LC-MS (ESI) m/z 225 [M + H]+; Hi-Res LC-MS (ESI) m/z calcd. for C12H16O4 [M + H]+ 225.1121, found 225.1120.

2.4.2. [4-[(Diethylcarbamoyl) methoxy]-3-methoxyphenyl] Acetic Acid Propyl Ester (Propanidid)

3-Methoxy-4-hydroxyphenylacetic acid propyl ester (2) (3.4 mmol, 0.8 g) was dissolved in acetone (0.27 M, 20 mL). Potassium carbonate (5.1 mmol, 705 mg) was added to the solution, followed by 2-chloro-N,N-diethylacetamide (4.0 mmol, 0.55 mL). Under vigorous stirring, the suspension was warmed to reflux (60 °C) for 16 h. After cooling to room temperature, the reaction mixture was filtered and the solvent removed under reduced pressure. The product was purified by silica column chromatography (ethyl acetate:hexane) to afford the title compound as a pale yellow oil (0.64 g, 56%) (Figures S3 and S4). 1H-NMR (400 MHz, CDCl3) δ: ppm 6.88 (d, J = 8.2 Hz, 1H), 6.82 (d, J =1.4 Hz 1H), 6.77 (d, J = 8.2 Hz, 1H), 4.7 (s, 2H) 4.04–3.99 (m, 2H), 3.84 (s, 3H), 3.52 (s, 2H), 3.32–3.42 (m, 4H), 1.57–1.64 (m, 2H), 1.18–1.14 (m, 3H), 1.12–1.08 (m, 3H), 0.87–0.91 (t, J = 7.3 Hz, 3H); 13C-NMR (101 MHz, CDCl3) δ: ppm 170.7, 166.0, 148.4, 145.7, 127.0, 120.4, 113.2, 112.1, 67.4, 65.3, 54.7, 40.5, 39.8, 39.2, 21.0, 13.2, 11.8, 9.4; LC-MS (ESI) m/z 338 [M + H]+; Hi-Res LC-MS (ESI) m/z calcd. for C18H27NO5 [M + H]+ 338.1962, found 338.1967.

2.4.3. Entacapone

Entacapone (purchased from Sigma-Aldrich, Figure S5). 1H-NMR (400 MHz, CDCl3) δ: ppm 10.87 (s, 1H), 8.06–8.05 (d, J = 2.0 Hz, 1H), 7.83–7.82 (d, J = 2.0 Hz, 1H), 7.51 (s, 1H), 7.19 (s, 1H), 6.10 (s, 1H), 3.44 (s, 2H), 1.52 (s, 2H), 1.25–1.17 (m, 3H).

2.4.4. Lidocaine

Lidocaine (purchased from Sigma-Aldrich, Figure S6). 1H-NMR (400 MHz, CDCl3) δ: ppm 8.93 (br s, 1H) 7.10–7.09 (m, 3H) 3.22 (s, 2H) 2.66–2.71 (q, J = 7.0 Hz, 4H) 2.23 (s, 6H) 1.15–1.12 (t, J = 7.0 Hz, 6H).

3. Results

3.1. Comparison of Drug Metabolite OP to Parent Drug OP

The selected drugs’ oxidation potentials (reported, mean, and standardised OP to Normal Hydrogen Electrode (N.H.E.) where sufficient data are available) alongside the mean half-life measurements are shown in Table 1. It was found that drug metabolites tended to have lower OP than the parent drug molecules, including Diclolfenac (700 mV) vs. 4′-OH-DCL (167 mV) and 5′-OH-DCL (236 mV), Clozapine (475 mV) vs. Norclozapine (390 mV), and Flupirtine (443 mV) vs. D13223 (347 mV).

3.2. Relationship between Drug OP and Half-Life

The relationships between all drugs/metabolites’ OPs with half-life are shown in Figure 1a. The relationships of only those molecules the OP of which could be standardised to N.H.E. against mean half-life are shown in Figure 1b. In all cases, an inverse relationship between OP and half-life was observed.

3.3. Synthesis of Propanidid

The synthetic pathway to the short acting anaesthetic propanidid is shown in Scheme 1. Propanidid was synthesised using an adapted method (in two steps) [68]. Fischer esterification of the carboxylic acid (1) with propan-1-ol in a pressure tube afforded the desired ester (2) in an excellent isolated yield (86%). The reaction of the ester (2) with carbamoyl chloride afforded propanidid in a good isolated yield (56%) after chromatography.

3.4. Voltammetric Study of Propanidid, Entacapone and Lidocaine.

The additional short- and long-acting drugs propanidid, entacapone and lidocaine’s LSV measurements are shown in Figure 2a–c. Two clear Epox are observed at +1.15 V and +1.97 V for lidocaine (υ = 25 mV s−1), indicating that an ECE (electrical–chemical–electrical) reaction occurred.
The LSV of lidocaine indicates that an electrochemical oxidation process occurs through two consecutive electron-transfer processes separated by a chemical deprotonation—first an electrochemical oxidation and deprotonation to the amidyl radical and then a further oxidation to the corresponding cation. Gieshoff [69] has observed a similar oxidation mechanism with anilides.
The LSVs of propanidid and entacapone indicate that multiple electron transfer reactions are taking place. However, apart from the first Epox values that are clearly visible, the other oxidation peaks occur after 2.0 V, and are all broad and undefined.
Analysis of the LSV data in terms of log peak current (Ip) vs. log scan rate (mV s−1) for the three drugs was performed, revealing in all three cases that the gradient is close to 0.5, indicating that the electrochemical process is operating via a diffusional process (rather than adsorption) [70,71,72,73]. It is readily observable that the peak potential shifts to more positive potentials due to the electrochemical process being irreversible.

4. Discussion

4.1. Comparing Parent Drugs to Drug Metabolites

All retrieved drugs and metabolites data are shown in Table 1. Metabolic oxidation of diclofenac (DCL) gives the unsTable 4’-OH-DCL and 5-OH-DCL, which induce hepatoxicity and can be metabolised further. This is in accordance with the finding that the drug metabolites have lower oxidation potentials than diclofenac. 5-OH-DCL forms a GSH conjugate without NADPH, but 4′-OH-DCL relies on NADPH, which demonstrates that 5-OH-DCL is liable to auto-oxidation [25].
Clozapine undergoes first pass metabolism in the liver, mainly catalysed by CYP1A2. Clozapine increases interactions with other drugs, which induces severe adverse effects [74]. It is further metabolised to give Norclozapine and Clozapine N-oxide. The redox activities of Clozapine and its metabolite (Norclozapine) are similar [27].
Flupirtine metabolism gives glucuronides and mercapturic acid derivatives (via the carbamate), which are unstable and toxic. A reactive diamine radical intermediate is formed which is toxic to cells and genes [75]. Further metabolism affords D13223. D13223 is a major active metabolite of Flupirtine and is further metabolised. Compared to Flupirtine, D13223 is easily oxidised, but the reduction is more difficult [76].
It was found that drug metabolites had lower OP vlaues than the parent drug. For example, 4′-OH-DCL and 5-OH-DCL, Norclozapine and D13223 have lower OP values than their parent drugs Diclofenac, Clozapine and Flupirtine, respectively (Table 1). Furthermore, these metabolites were more toxic than the parent drug. Among the drug metabolites generated from the same parent drug, metabolites with lower OP are highly toxic compared to others (i.e., 4′-OH-DCL is more toxic than 5-OH-DCL).

4.2. Drug Metabolism Pathways

Oxidation potential (OP) can inform the ability of the compounds to generate reactive oxygen species (ROS), and therefore the oxidative stress induction [77]. More than one measurement of OP value was collected for some compounds (i.e., Paracetamol, Diclofenac, Trimethoprim, Clozapine and Flupirtine) where available. Secondly, the half-life is an important parameter to assess drug stability alongside hepatic clearance. A less stable drug is intensively metabolised, and both half-life and clearance rate are responsible for drug elimination [78]. A drug metabolite with a longer half-life is the major (most stable) product, although it is not always safe, as it implies drugs will stay in the body for an extended period before being removed. Hepatoxicity is induced because of the reactive intermediate induced by the metabolism processes. In most cases, metabolites are responsible for toxicity due to bioactivation [79].
Although the plot appeared to be random before standardising OP (Figure 1a), a stronger correlation was seen for the standardised (std.) OP (Figure 1b). As shown in Figure 1a, the peak of half-life was seen at 250–500 mV. This newly identified inverse correlation trend, whereby a lower OP has a longer half-life and vice versa, implies that a compound with a lower OP is more biologically stable, rather than reactive. However, increasing the biological stability of a drug implies metabolites (with a lower OP) may stay in the body longer.
A multitude of factors affect a drug’s pharmacology, including stability and toxicity. Clearly, predicting all the drug’s interactions in the body from one factor such as OP is not possible, however a rapid screen of new drug entities’ (or during hit-to-lead development) OP values would give insight into the much later biological behaviour of the compound under study, and provide new knowledge to guide the intelligent design of improved drug molecules.
Due to the limited number of drugs that have both a reported OP and half-life in the literature, a statistical analysis to determine if this inversely proportional trend between OP and half-life is valid would not be relevant.
We therefore looked at the respective drug metabolism pathways for further insight. Promazine is a metabolite of Chlorpromazine [80]. More than 80% of Promethazine is absorbed in the body, and metabolism is via first-pass (liver), glucuronidation and sulfation [81].
Acebutolol is metabolised in the liver to give hepatoxic (active) metabolites such as diacetolol, hydroxylamine and ‘auto-oxidised’ metabolites, which are responsible for toxicity [82]. Hydrolysis forms an arylamine, and the CYP450 oxidation of arylamine induces toxicity [82].
The organometallic 1,1′-ferrocene dimethanol is not actively metabolised.
Warfarin is metabolised by the liver in a regio- and stereo-selective manner. Oxidation gives hydroxywarfarin which accounts for up to 85% of warfarin’s metabolites [83]. S- and R-warfarin are metabolised by different CYP450 isoforms, including CYP2C19 and CYP2C8 (with CYP2C19), respectively [83]. S-warfarin has a shorter half-life (mean 32 h) than R-warfarin (mean 58 h) [30].
The metabolism of Paracetamol is responsible for both its therapeutic effects and toxicity (hepatoxicity) [84]. Reactive metabolites such as the quinone are rapidly formed. Biotransformation is mainly a detoxification processes, but N-acetyl-p-benzoquinone imine (NAPQI) is responsible for hepatocyte cell death due to GSH’ depletion’ [85].
Only 10–20% of Trimethoprim is metabolised in the liver [86]. Children have a rapid clearance rate, three times greater than adults [87].
Amodiaquine is metabolised in the liver and neutrophils to give a metabolite (menaquinone) [88]. Accumulating metabolites increases free haem level-induced toxicity [89].
Caffeine is metabolised in the liver to give Theobromine, Paraxanthine (major) and Theophylline [90]. The cardiovascular, respiratory, renal and nervous systems are affected by caffeine intake [91].
The reaction mechanisms for Carisoprodol have not been entirely elucidated [49]. CYP2C19 is responsible for hepatic biotransformation [92].
It can be seen that the available drugs in the literature that have both oxidation potential and stability measurements (half-life) have a wide range of different metabolic pathways, thus permitting confidence in the inverse relationship between measured OP and drug half-life.

4.3. New Drug OP Measurements and Stability Inference

Next, we applied the knowledge gained from the data-mining experiment to previously unreported bioactive molecules to expand the data set and test the finding of an inverse relationship between OP and half-life. An under-represented drug class in the original data set is the short half-life drugs.
Propanidid was selected as a short-acting phenylacetate general anaesthetic, which has been withdrawn from the market due to its side effect of causing anaphylactic reactions. The primary metabolite for Propanidid is (4-(2-[diethylamino]-2-oxoethoxy)-3-methoxy-benzeneacetic acid (DOMA) [93]. To the best of our knowledge, no studies in the literature have reported any electrochemical data for propanidid.
Entacapone is used in the treatment of Parkinson’s disease in combination with other drugs. The primary metabolite is the glucuronide formed in the liver, and the remaining 5% is converted to the Z-isomer [94]. Several electrochemical methods have been reported in the literature for the detection of the metabolites [95,96,97].
Lidocaine is a local anaesthetic and is one of the most studied drugs for both the detection and isolation of a drug metabolite using electrochemical methods [98,99,100,101,102]. The major metabolite of lidocaine is the N-deethylated form.
We found that OP has an inverse relationship with the drug’s stability in the body (half-life), with 1450 mV, 1250 mV and 1150 mV correlating to 5.9 min [65], 24 min [66] and 87–108 min [67], for propanidid, entacapone and lidocaine, respectively.

4.4. Study limitations

The limitations of the study include the following: the number of available drug molecules that have robust voltammetry information combined with half-life measurements; a lack of standardisation in the literature of electrodes (including working electrode surface area), electrolytes, solvents (including concentration), and the pH of the cell, which could lead to subtle differences in the measured oxidation potential via voltammetry (however, where reported, the reference electrode measurement could be standardised to an N.H.E.); the half-life of drugs in the body depends on a number of patient characteristics (age, sex, diet, underlying health conditions), thus a range of half-life measurements are to be expected.

5. Conclusions

Using a combination of data mining of available oxidation potential (OP) and biological stability measurements from the literature, a diverse range of drugs (and metabolites) were identified. Standardising the OP to N.H.E. and the comparison of inter-species stability measurements identified an unanticipated inverse relationship between lower OP and higher stability in vivo for the first time.
This result in part supports our original hypothesis that oxidation potential correlates with drug stability, however it conflicts with our original assumption that lower oxidation potential compounds would be more readily cleared.
A prospective survey of short-acting drug molecules, including their synthesis and characterisation, and a detailed voltammetric study indicated an emerging inverse trend between standardised OP and circulating half-life. These findings demonstrate that OP measurements hold promise as a new tool for rapidly predicting the pharmacokinetic (PK) properties of a new chemical entity for drug discovery campaigns.

Supplementary Materials

The following are available online at, Figure S1: 1H NMR spectrum of 2 (400 MHz, CDCl3); Figure S2. 13C NMR spectrum of 2 (101 MHz, CDCl3); Figure S3: 1H NMR spectrum of Propanidid 400 MHz, CDCl3); Figure S4: 13C NMR spectrum of Propanidid (101 MHz, CDCl3); Figure S5: 1H NMR spectrum of Entacapone (400 MHz, CDCl3); Figure S6: 1H NMR spectrum of Lidocaine (400 MHz, CDCl3).

Author Contributions

Conceptualization, H.F. and A.M.J.; Methodology, H.F. and M.K.B.; Validation, H.F., A.M.J., M.K.B., D.A.C.B. and C.E.B.; Formal Analysis, H.F. and A.M.J.; Investigation, H.F. and M.K.B.; Data Curation, H.F. and M.K.B.; Writing—Original Draft Preparation, H.F., A.M.J. and M.K.B.; Writing—Review and Editing, H.F., A.M.J., D.A.C.B., and C.E.B.; Visualization, H.F. and M.K.B.; Supervision, A.M.J., D.A.C.B. and C.E.B.; Project Administration, A.M.J. and C.E.B. All authors have read and agreed to the published version of the manuscript.


This research received no external funding.


The authors thank the analytical staff at MMU (UK) for NMR and mass-spectrometry analyses.

Conflicts of Interest

The authors declare no conflict of interest.


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Figure 1. (a) Relationship between OP and half-life of all compounds; (b) Relationship between standardised OP (N.H.E.) and half-life. Key: * = additional drugs studied and number after the drug indicates which entries from Table 1 were standardized.
Figure 1. (a) Relationship between OP and half-life of all compounds; (b) Relationship between standardised OP (N.H.E.) and half-life. Key: * = additional drugs studied and number after the drug indicates which entries from Table 1 were standardized.
Scipharm 88 00046 g001
Scheme 1. Adapted synthesis of propanidid to enable the voltammetric study.
Scheme 1. Adapted synthesis of propanidid to enable the voltammetric study.
Scipharm 88 00046 sch001
Figure 2. The LSV of the compounds using glassy carbon electrode (GCE) as the working electrode (WE), platinum as the counter electrode (CE) and Ag/AgCl as the reference electrode(RE). TBAP (0.5 M), MeCN: MeOH (10:1). (a) Propanidid, (b) entacapone, and (c) lidocaine.
Figure 2. The LSV of the compounds using glassy carbon electrode (GCE) as the working electrode (WE), platinum as the counter electrode (CE) and Ag/AgCl as the reference electrode(RE). TBAP (0.5 M), MeCN: MeOH (10:1). (a) Propanidid, (b) entacapone, and (c) lidocaine.
Scipharm 88 00046 g002
Table 1. Oxidation potential (OP) and half-life of identified compounds.
Table 1. Oxidation potential (OP) and half-life of identified compounds.
Drug/CompoundOP (mV)Mean OP (mV)Std. OP (mV)Mean t1/2 (h)Reference (s)
Scipharm 88 00046 i001
167--4.3OP [25]
t1/2 [26]
Scipharm 88 00046 i002
236--2.5OP [25]
t1/2 [26]
Scipharm 88 00046 i003
261--48.0OP [27]
t1/2 [27]
Scipharm 88 00046 i004
280-48540.0OP [28]
t1/2 [29,30]
Scipharm 88 00046 i005
347-63510.7OP [31]
t1/2 [27]
Scipharm 88 00046 i006
390--22.5OP [27]
t1/2 [32]
Flupirtine 1
Scipharm 88 00046 i007
535442.58238.0OP [31,33]
t1/2 [34,35]
Flupirtine 2350638
Clozapine 1
Scipharm 88 00046 i008
600475.3-11.9OP [11,25,27,36,37]
t1/2 [32,38,39,40]
Clozapine 2440645
Clozapine 3381-
Clozapine 4480-
Scipharm 88 00046 i009
530-73530.0OP [4]
t1/2 [41]
Paracetamol 1
Scipharm 88 00046 i010
392595.5-2.2OP [5,25,42,43]
t1/2 [44,45,46]
Paracetamol 2400-
Paracetamol 3610898
Paracetamol 49801185
Scipharm 88 00046 i011
670-8759.5OP [4]
t1/2 [47]
Diclofenac 1
Scipharm 88 00046 i012
870700.011581.7OP [48,49,50]
t1/2 [26,51,52]
Diclofenac 2630827
Diclofenac 3600799
Trimethoprim 1
Scipharm 88 00046 i013
1000950.0-9.0OP [5,53]
t1/2 [54]
Trimethoprim 2900-
Scipharm 88 00046 i014
970--5.1OP [55]
t1/2 [56,57]
Scipharm 88 00046 i015
1002--9.0OP [58]
t1/2 [59]
Scipharm 88 00046 i016
1500-17054.6OP [60]
t1/2 [61,62]
Scipharm 88 00046 i017
2003-22082.0OP [43]
t1/2 [63,64]
Additional drugs studied
Scipharm 88 00046 i018
1450-16550.1 (5.9 min)t1/2 [65]
Scipharm 88 00046 i019
1250-14550.4 (24 min)t1/2 [66]
Scipharm 88 00046 i020
1150-13551.6 (97.5 min)t1/2 [67]
Compounds with sufficient data (e.g., concentration of electrolytes, reference electrode used) enabled the calculation of the standardised (std.) OP to the normal hydrogen electrode (N.H.E.).

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Fuchigami, H.; Bal, M.K.; Brownson, D.A.C.; Banks, C.E.; Jones, A.M. Voltammetric Behaviour of Drug Molecules as a Predictor of Metabolic Liabilities. Sci. Pharm. 2020, 88, 46.

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Fuchigami H, Bal MK, Brownson DAC, Banks CE, Jones AM. Voltammetric Behaviour of Drug Molecules as a Predictor of Metabolic Liabilities. Scientia Pharmaceutica. 2020; 88(4):46.

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Fuchigami, Hikari, Mandeep K. Bal, Dale A. C. Brownson, Craig E. Banks, and Alan M. Jones. 2020. "Voltammetric Behaviour of Drug Molecules as a Predictor of Metabolic Liabilities" Scientia Pharmaceutica 88, no. 4: 46.

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