A New DNA Repair-Related Platform for Pharmaceutical Outlook in Cancer Therapies: Ultrashort Single-Stranded Polynucleotides
by
Sergey Stovbun 1, Kirill Ermakov 2,*, Alexander Bukhvostov 2, Alexander Vedenkin 1 and Dmitry Kuznetsov 1,2,*
Sergey Stovbun 1, Kirill Ermakov 2,*, Alexander Bukhvostov 2, Alexander Vedenkin 1 and Dmitry Kuznetsov 1,2,*
1
N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Kosygin St., 4, Moscow 119991, Russia
2
School of Biomedicine, N.I. Pirogov Russian National Research Medical University, Ostrovityanov St., 1, Moscow 117997, Russia
*
Authors to whom correspondence should be addressed.
Sci. Pharm. 2019, 87(4), 25; https://doi.org/10.3390/scipharm87040025
Received: 6 August 2019 / Revised: 30 September 2019 / Accepted: 3 October 2019 / Published: 5 October 2019
Thio- and cyano- modified single-stranded poly(dNTP) sequences of different molecular sizes (20–200 n) and the same lengths routine poly(dNTP) and poly(NTP) species were tested for their impact on catalytic activities of β-like DNA polymerases from chromatin of HL-60, WERI-1A and Y-79 cells as well as for the affinity patterns in DNApolβ-poly(dNTP)/(NTP) pairs, respectively. An essential link between the lengths of ultrashort (50–100 n) single-stranded poly(dNTP) sequences of different structures and their inhibitory effects towards the cancer-specific DNA polymerases β was found. A possible significance of this phenomenon for both DNA repair suppression in tumors and a consequent anti-cancer activity of the DNA repair related short poly(dNTP) fragments is under discussion.
Keywords:
magnetic isotope effects (MIE); DNA repair; DNA polymerases; polydeoxyribonucleotides (PDRN), PDRN – enzyme binding
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MDPI and ACS Style
Stovbun, S.; Ermakov, K.; Bukhvostov, A.; Vedenkin, A.; Kuznetsov, D. A New DNA Repair-Related Platform for Pharmaceutical Outlook in Cancer Therapies: Ultrashort Single-Stranded Polynucleotides. Sci. Pharm. 2019, 87, 25.
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