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Sci. Pharm. 2017, 85(1), 3;

Transmission Electron Microscopy of XDR Mycobacterium tuberculosis Isolates Grown on High Dose of Ofloxacin

Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak 3848176941, Iran
Department of Biology, Payame Noor University, Tehran 79134-13915, Iran
Research Institute for Epidemiology and Microbiology, Minsk 220114, Belarus
Institute for Pulmonology and Phthisiology, Minsk 220114, Belarus
Author to whom correspondence should be addressed.
Received: 23 September 2016 / Accepted: 3 January 2017 / Published: 2 February 2017
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The aim of the study was to investigate behavior of resistant Mycobacterium tuberculosis (MTB) isolates under a high dose of ofloxacin and its morphological changes. 19 extensively drug resistant (XDR) clinical isolates of MTB were grown on Löwenstein–Jensen medium containing progressively increasing concentrations of ofloxacin (2, 4, 8, 16, 32 mg/L). Ultra-structure analyses of resistant isolates grown on ofloxacin were conducted by transmission electron microscopy (TEM). Fixation was carried out by 4% glutaraldehyde in 0.1 M sodium cacodylate buffer on 300 mesh carbon formvar copper grid. The samples were negatively stained with uranium acetate suspension. All19XDRMTBisolatesweregrownandformedcoloniessuccessfullyon2,4,8mg/L,sevenisolates on16mg/L,andfourisolateson32mg/Lofloxacin. Morphologicalchangesandunusualformswere detected in 8, 16 and 32 mg/L ofloxacin at 43%, 76.5% and 81% of cells, respectively. Swollen form (protoplast like), ghost-like cell, degraded forms, and in a few cases, detached cytoplasm from cell wall were clearly detected in high drug concentrations in comparison to control. Changes in morphology were increased with increasing ofloxacin concentrations (p < 0.05). Some XDR isolates could be successfully grown on high doses of ofloxacin (32 mg/L), but with changes in morphology. It was concluded that several magnitudes of the drug doses could not prevent growth of drug resistant forms. View Full-Text
Keywords: Mycobacterium tuberculosis; transmission electron microscope; XDR; ofloxacin Mycobacterium tuberculosis; transmission electron microscope; XDR; ofloxacin

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Arjomandzadegan, M.; Sadrnia, M.; Titov, L.; Surkova, L.; Sarmadian, H.; Ghasemikhah, R.; Hosseiny, H. Transmission Electron Microscopy of XDR Mycobacterium tuberculosis Isolates Grown on High Dose of Ofloxacin. Sci. Pharm. 2017, 85, 3.

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