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Sci. Pharm.
Volume 83
Issue 2
10.3797/scipharm.1501-12
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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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Article

Suppression of IgM Proteolysis by Conformational Stabilization Through Excipients

by
Monika MUELLER
1,2,*,
Maybelle Q. T. LOH
2 and
Pete GAGNON
2
1
Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
2
Bioprocessing Technology Institute, A*STAR, 20 Biopolis Way, #06-01, Centros, 138668, Singapore.
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2015, 83(2), 401-410; https://doi.org/10.3797/scipharm.1501-12
Submission received: 21 January 2015 / Accepted: 4 March 2015 / Published: 4 March 2015
Versions Notes

Abstract

Protease activity from host cell lines may cause product loss or affect the quality of recombinant proteins. In this study, we showed that excipients like glycine and sorbitol reduce the proteolysis of an immunoglobulin M (IgM) in the presence of added proteases like α-chymotrypsin, papain, and pepsin. The activity of the proteases in the IgM-protective environments was conserved or even enhanced as tested using low molecular weight substrates. Thus, a higher resistance against proteolytic degradation appears to be caused by the conformational stabilization of the IgM due to preferential exclusion of sorbitol and glycine.
Keywords: IgM; Proteolysis; Conformational stability; Sorbitol; Glycine IgM; Proteolysis; Conformational stability; Sorbitol; Glycine

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MDPI and ACS Style

MUELLER, M.; LOH, M.Q.T.; GAGNON, P. Suppression of IgM Proteolysis by Conformational Stabilization Through Excipients. Sci. Pharm. 2015, 83, 401-410. https://doi.org/10.3797/scipharm.1501-12

AMA Style

MUELLER M, LOH MQT, GAGNON P. Suppression of IgM Proteolysis by Conformational Stabilization Through Excipients. Scientia Pharmaceutica. 2015; 83(2):401-410. https://doi.org/10.3797/scipharm.1501-12

Chicago/Turabian Style

MUELLER, Monika, Maybelle Q. T. LOH, and Pete GAGNON. 2015. "Suppression of IgM Proteolysis by Conformational Stabilization Through Excipients" Scientia Pharmaceutica 83, no. 2: 401-410. https://doi.org/10.3797/scipharm.1501-12

APA Style

MUELLER, M., LOH, M. Q. T., & GAGNON, P. (2015). Suppression of IgM Proteolysis by Conformational Stabilization Through Excipients. Scientia Pharmaceutica, 83(2), 401-410. https://doi.org/10.3797/scipharm.1501-12

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