Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2015).
Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence.
Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
Sci. Pharm. 2015, 83(2), 401-410; https://doi.org/10.3797/scipharm.1501-12 (registering DOI)
Suppression of IgM Proteolysis by Conformational Stabilization Through Excipients
Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
Bioprocessing Technology Institute, A*STAR, 20 Biopolis Way, #06-01, Centros, 138668, Singapore.
Author to whom correspondence should be addressed.
Received: 21 January 2015 / Accepted: 4 March 2015 / Published: 4 March 2015
PDF [139 KB, uploaded 8 September 2016]
Abstract Protease activity from host cell lines may cause product loss or affect the quality of recombinant proteins. In this study, we showed that excipients like glycine and sorbitol reduce the proteolysis of an immunoglobulin M (IgM) in the presence of added proteases like α-chymotrypsin, papain, and pepsin. The activity of the proteases in the IgM-protective environments was conserved or even enhanced as tested using low molecular weight substrates. Thus, a higher resistance against proteolytic degradation appears to be caused by the conformational stabilization of the IgM due to preferential exclusion of sorbitol and glycine.
This is an open access article distributed under the Creative Commons Attribution License
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
Share & Cite This Article
MDPI and ACS Style
MUELLER, M.; LOH, M.Q.T.; GAGNON, P. Suppression of IgM Proteolysis by Conformational Stabilization Through Excipients. Sci. Pharm. 2015, 83, 401-410.
MUELLER M, LOH MQT, GAGNON P. Suppression of IgM Proteolysis by Conformational Stabilization Through Excipients. Scientia Pharmaceutica. 2015; 83(2):401-410.
MUELLER, Monika; LOH, Maybelle Q.T.; GAGNON, Pete. 2015. "Suppression of IgM Proteolysis by Conformational Stabilization Through Excipients." Sci. Pharm. 83, no. 2: 401-410.
Show more citation formats
Show less citations formats
[Return to top]
For more information on the journal statistics, click here
Multiple requests from the same IP address are counted as one view.