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Volume 82
Issue 3
10.3797/scipharm.1402-19
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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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Article

Development and Validation of a Liquid Chromatography-Mass Spectrometry Method for the Determination of Zileuton in Human Plasma

by
Katakam PRAKASH
1,
Shanta K. ADIKI
1 and
Rama Rao KALAKUNTLA
2,*
1
Nirmala College of Pharmacy, Mangalagiri, Guntur 522503, AP, India
2
Department of Biotechnology, Acharya Nagarjuna University, Guntur, AP, India
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2014, 82(3), 571-584; https://doi.org/10.3797/scipharm.1402-19
Submission received: 27 February 2014 / Accepted: 26 April 2014 / Published: 26 April 2014
Versions Notes

Abstract

A selective and sensitive liquid chromatography-tandem mass spectrometric method (LC-MS/MS) has been developed and validated for the quantification of zileuton in human plasma. Deuterated internal standard (zileuton D4) was used as the internal standard (ISTD). Zileuton was extracted by liquid-liquid extraction using methyl tert-butyl ether and separated by isocratic elution on a C18 column (100 x 4.6 mm, 5 μm, Discovery C18) with the mobile phase consisting of 1 mM ammonium acetate buffer and methanol in the ratio of 10:90. A flow rate of 1.0 ml/min was used with isocratic elution. Multiple reaction monitoring transitions in positive mode for zileuton and the internal standard were 237.3/161.2 and 241.2/161.1, respectively. The method was validated within the linearity range of 50.5–10,012.7 ng/ml for the bioanalytical method validation parameters like selectivity, accuracy, precision, recovery, stability, and matrix effect.
Keywords: Zileuton; Plasma; LC-MS/MS; Validation Zileuton; Plasma; LC-MS/MS; Validation

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MDPI and ACS Style

PRAKASH, K.; ADIKI, S.K.; KALAKUNTLA, R.R. Development and Validation of a Liquid Chromatography-Mass Spectrometry Method for the Determination of Zileuton in Human Plasma. Sci. Pharm. 2014, 82, 571-584. https://doi.org/10.3797/scipharm.1402-19

AMA Style

PRAKASH K, ADIKI SK, KALAKUNTLA RR. Development and Validation of a Liquid Chromatography-Mass Spectrometry Method for the Determination of Zileuton in Human Plasma. Scientia Pharmaceutica. 2014; 82(3):571-584. https://doi.org/10.3797/scipharm.1402-19

Chicago/Turabian Style

PRAKASH, Katakam, Shanta K. ADIKI, and Rama Rao KALAKUNTLA. 2014. "Development and Validation of a Liquid Chromatography-Mass Spectrometry Method for the Determination of Zileuton in Human Plasma" Scientia Pharmaceutica 82, no. 3: 571-584. https://doi.org/10.3797/scipharm.1402-19

APA Style

PRAKASH, K., ADIKI, S. K., & KALAKUNTLA, R. R. (2014). Development and Validation of a Liquid Chromatography-Mass Spectrometry Method for the Determination of Zileuton in Human Plasma. Scientia Pharmaceutica, 82(3), 571-584. https://doi.org/10.3797/scipharm.1402-19

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