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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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Sci. Pharm. 2012, 80(2), 433-446; (registering DOI)

Characterization of Constituents and Anthelmintic Properties of Hagenia abyssinica

Leibniz Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle (Saale), Germany
Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland
University of Basel, P.O. Box, CH-4003 Basel, Switzerland
University of Addis Ababa, P.O. Box 30270, Addis Ababa, Ethiopia
Author to whom correspondence should be addressed.
Received: 16 September 2011 / Accepted: 2 March 2012 / Published: 2 March 2012
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The dried female flowers of Hagenia abyssinica (Bruce) J. F. Gmel. (Rosaceae) are traditionally used as an anthelmintic remedy in Ethiopia and formerly were incorporated into the European Pharmacopoeia. One-, two- and tricyclic phloroglucinol derivatives (kosins) were suggested to be the active principles. However, polar constituents may also contribute to the activity. Therefore, we investigated for the first time the polar constituents. We isolated typical Rosaceae constituents such as quercetin 3-O-β-glucuronide, quercetin 3-O-β-glucoside and rutin. Polar kosin glycosides or derivatives could not be detected.
The anthelmintic activity of fractions of different polarity were tested against the blood fluke Schistosoma mansoni, the liver flukes Clonorchis sinensis and Fasciola hepatica and the intestinal fluke Echinostoma caproni. The anthelmintic activity decreased with increasing polarity of the tested fractions. ESI-MS investigations indicated the predominant occurrence of kosins in the active fractions.
Using the anthelmintic active extracts of Hagenia abyssinica we developed a simple, inexpensive bioassay against the non-parasitic nematode Caenorhabditis elegans, which can be used as an initial screening procedure for anthelmintic properties of crude extracts of plants or fungi. The anthelmintic activity of test extracts against the model organism was determined in a microtiter plate assay by enumeration of living and dead nematodes under a microscope.
Keywords: Chaenorhabditis elegans; Trematodes; Bioassay; Kosso; Bioactivity; Kosins Chaenorhabditis elegans; Trematodes; Bioassay; Kosso; Bioactivity; Kosins
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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THOMSEN, H.; REIDER, K.; FRANKE, K.; WESSJOHANN, L.A.; KEISER, J.; DAGNE, E.; ARNOLD, N. Characterization of Constituents and Anthelmintic Properties of Hagenia abyssinica. Sci. Pharm. 2012, 80, 433-446.

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