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Volume 79
Issue 3
10.3797/scipharm.1105-13
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Article

Validated RP-HPLC Method for Simultaneous Quantitation of Losartan Potassium and Metolazone in Bulk Drug and Formulation

by
Ramkumar DUBEY
,
Vidhya K. BHUSARI
and
Sunil R. DHANESHWAR
*
Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Pune, Maharashtra, 411038, India
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2011, 79(3), 545-554; https://doi.org/10.3797/scipharm.1105-13
Submission received: 16 May 2011 / Accepted: 26 June 2011 / Published: 26 June 2011
Versions Notes

Abstract

A HPLC method has been described for simultaneous determination of Losartan potassium and Metolazone in formulation. This method is based on a HPLC separation of the two drugs on the Thermo Hypersil BDS–C18 (250 mm × 4.6 mm, 5.0 μm) with isocratic conditions and a simple mobile phase containing acetonitrile:water (60:40) at a flow rate of 0.8 mL/min using UV detection at 237 nm. This method has been applied to a marketed formulation without interference of excipients. The linear regression analysis data for the calibration plots showed a good linear relationship over the concentration range of 2–12 μg/mL for Losartan potassium and 0.2–1.2 μg/mL for Metolazone, respectively. The method was validated for precision, robustness and recovery. Statistical analysis showed that the method is repeatable and selective for the estimation of Losartan potassium and Metolazone.
Keywords: Losartan potassium; Metolazone; HPLC; Validation; Metoz© Losartan potassium; Metolazone; HPLC; Validation; Metoz©

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MDPI and ACS Style

DUBEY, R.; BHUSARI, V.K.; DHANESHWAR, S.R. Validated RP-HPLC Method for Simultaneous Quantitation of Losartan Potassium and Metolazone in Bulk Drug and Formulation. Sci. Pharm. 2011, 79, 545-554. https://doi.org/10.3797/scipharm.1105-13

AMA Style

DUBEY R, BHUSARI VK, DHANESHWAR SR. Validated RP-HPLC Method for Simultaneous Quantitation of Losartan Potassium and Metolazone in Bulk Drug and Formulation. Scientia Pharmaceutica. 2011; 79(3):545-554. https://doi.org/10.3797/scipharm.1105-13

Chicago/Turabian Style

DUBEY, Ramkumar, Vidhya K. BHUSARI, and Sunil R. DHANESHWAR. 2011. "Validated RP-HPLC Method for Simultaneous Quantitation of Losartan Potassium and Metolazone in Bulk Drug and Formulation" Scientia Pharmaceutica 79, no. 3: 545-554. https://doi.org/10.3797/scipharm.1105-13

APA Style

DUBEY, R., BHUSARI, V. K., & DHANESHWAR, S. R. (2011). Validated RP-HPLC Method for Simultaneous Quantitation of Losartan Potassium and Metolazone in Bulk Drug and Formulation. Scientia Pharmaceutica, 79(3), 545-554. https://doi.org/10.3797/scipharm.1105-13

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