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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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Sci. Pharm. 2009, 77(3), 567-578; (registering DOI)

Preparation and Biological Evaluation of a [55Co]-2-Acetylpyridine Thiosemicarbazone

Agricultural, Medical and Industrial Research School (AMIRS), Nuclear Science and Technology Research Institute (NSTRI) Karaj, P.O.Box: 31485-498, Iran
Author to whom correspondence should be addressed.
Received: 4 July 2009 / Accepted: 3 August 2009 / Published: 5 August 2009
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Due to the anti-proliferative properties of cobalt-thiosemicarbazone complexes, the production of [55Co](III)-bis-(2-acetylpyridine thiosemicarbazone) ([55Co](III)[APTS]2) was investigated. Co-55 (T1/2=17.53 h) was produced by 150 μA irradiation of a natural nickel target by 15 MeV protons. The 55Co was separated from the irradiated target material using a two-step method with a radiochemical yield of >95% followed by radionuclidic and chemical purity control. [55Co](III)chloride was mixed with 2-acetylpyridine thiosemicarbazone for 30 min at room temperature to yield [55Co](III)[APTS]2 (radiochemical purity > 98% shown by RTLC/HPLC). A specific activity of about 10–20 Ci/mmol was obtained. The final solution was diluted in normal saline to 5% ethanolic solution for biological evaluation. The stability of the final product was checked in the absence and presence of human serum at 37°C to 24 h. The partition co-efficient of the final complex at the pH of 7 was 1.00±0.08. A significant tumor accumulation (%ID/g; 3.5%) was observed in tumoral tissue 21 h post injection in fibrosarcoma-bearing mice by biodistribution studies. Co-incidence imaging also demonstrated tumor uptake from 21–35 h however at 35 h tumor uptake is more specific and significant.
Keywords: Cobalt-55; Thiosemicarbazone; Biodistribution; Tumor imaging; Co-incidence; Fibrosarcoma Cobalt-55; Thiosemicarbazone; Biodistribution; Tumor imaging; Co-incidence; Fibrosarcoma
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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JALILIAN, A.R.; ROWSHANFARZAD, P.; AKHLAGHI, M.; SABET, M.; KAMALI-DEHGHAN, M.; POULADI, M. Preparation and Biological Evaluation of a [55Co]-2-Acetylpyridine Thiosemicarbazone. Sci. Pharm. 2009, 77, 567-578.

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