Next Article in Journal
Isolation and Characterization of an Impurity Obtained During the Synthesis of the Antibiotic Drug Sparfloxacin
Previous Article in Journal
Insight into the Structural Requirement of 2-Alkyl-4-(biphenylmethoxy)quinolines as Nonpeptide Angiotensin II Receptor Antagonists: A QSAR Approach
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Sci. Pharm.
Volume 77
Issue 1
10.3797/scipharm.0809-04
scipharm-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Development of a Sensitive and Stereoselective HPLC Method for the Analysis of Pindolol in Plasma and Pharmaceutical Products Using a Chiralpak IB Column and Fluorescence Detection

by
Mohamed HEFNAWY
*,
Mohamed KASSEM
,
Haba ABDINE
and
Nourah AL-ZOMAN
College of Pharmacy, Department of Pharmaceutical Chemistry, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2009, 77(1), 47-66; https://doi.org/10.3797/scipharm.0809-04
Submission received: 13 September 2008 / Accepted: 9 December 2008 / Published: 18 December 2008
Versions Notes

Abstract

A sensitive and stereoselective HPLC method has been developed for the simultaneous determination of pindolol enantiomers in plasma and pharmaceutical products. Enantiomeric resolution was achieved on a cellulose tris(3,5-dimethylphenylcarbamate) immobilized onto a 5 μm spherical porous silica chiral stationary phase (CSP) known as Chiralpak IB with fluorescence detection set at 266 nm for excitation and 308 nm for emission. The mobile phase consists of n-hexane–isopropanol–triethylamine, (50:50:0.5), (v/v/v) has been used at a flow rate of 1.0 ml/min. The method was highly specific where other coformulated drugs such as clopamide and isosorbide did not interfere. The stability of pindolol enantiomers under different degrees of temperature was studied. The method validated for its linearity, accuracy, precision and robustness. There was no significant difference (p>0.05) between inter- and intra-day studies for each enantiomers which confirmed the reproducibility of the assay method. Preliminary data suggest that S-(−)-pindolol induces less bradycardia but more sedation and central nervous system depression than racemic pindolol.
Keywords: Pindolol enantiomers; Chiralpak IB column; Plasma; Pharmaceutical formulations Pindolol enantiomers; Chiralpak IB column; Plasma; Pharmaceutical formulations

Share and Cite

MDPI and ACS Style

HEFNAWY, M.; KASSEM, M.; ABDINE, H.; AL-ZOMAN, N. Development of a Sensitive and Stereoselective HPLC Method for the Analysis of Pindolol in Plasma and Pharmaceutical Products Using a Chiralpak IB Column and Fluorescence Detection. Sci. Pharm. 2009, 77, 47-66. https://doi.org/10.3797/scipharm.0809-04

AMA Style

HEFNAWY M, KASSEM M, ABDINE H, AL-ZOMAN N. Development of a Sensitive and Stereoselective HPLC Method for the Analysis of Pindolol in Plasma and Pharmaceutical Products Using a Chiralpak IB Column and Fluorescence Detection. Scientia Pharmaceutica. 2009; 77(1):47-66. https://doi.org/10.3797/scipharm.0809-04

Chicago/Turabian Style

HEFNAWY, Mohamed, Mohamed KASSEM, Haba ABDINE, and Nourah AL-ZOMAN. 2009. "Development of a Sensitive and Stereoselective HPLC Method for the Analysis of Pindolol in Plasma and Pharmaceutical Products Using a Chiralpak IB Column and Fluorescence Detection" Scientia Pharmaceutica 77, no. 1: 47-66. https://doi.org/10.3797/scipharm.0809-04

APA Style

HEFNAWY, M., KASSEM, M., ABDINE, H., & AL-ZOMAN, N. (2009). Development of a Sensitive and Stereoselective HPLC Method for the Analysis of Pindolol in Plasma and Pharmaceutical Products Using a Chiralpak IB Column and Fluorescence Detection. Scientia Pharmaceutica, 77(1), 47-66. https://doi.org/10.3797/scipharm.0809-04

Article Metrics

Back to TopTop