Next Article in Journal
Synthesis and Preclinical Studies of [61Cu]-N-(2-hydroxyacetophenone)glycinate as a Possible PET Radiopharmaceutical
Previous Article in Journal
Anticonvulsant and Sedative-Hypnotic Activities of N-Acetyl / Methyl Isatin Derivatives
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Sci. Pharm.
Volume 74
Issue 3
10.3797/scipharm.2006.74.99
scipharm-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7- nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

by
N. El-Enany
*,
F. Belal
and
M. Rizk
Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, 35516, Mansoura, Egypt
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2006, 74(3), 99-119; https://doi.org/10.3797/scipharm.2006.74.99
Submission received: 5 January 2006 / Accepted: 22 March 2006 / Published: 30 September 2006
Versions Notes

Abstract

A simple and sensitive kinetic spectrophotometric method was developed for the determination of isoxsuprine in pharmaceutical preparations. The method is based upon a kinetic investigation of the coupling reaction between isoxsuprine HCl and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in borate buffer of pH 7.8 for a fixed time of 30 min. The absorbance of the yellow coloured product was measured at 395 nm. The absorbance- concentration plot was rectilinear over the range of 2-20 μg mL-1 (r= 0.9994) with minimum detectability of 0.6 μg mL-1 (1.75 x10-6M). The different experimental parameters affecting the development and stability of the colour were carefully studied and optimized. The determination of isoxsuprine by the fixed-concentration and rate-constant methods is also feasible with the calibration equations obtained, but the fixed time method has been found to be more applicable. The proposed method was further applied to the determination of the drug in formulations. The results obtained were in good agreement with those obtained using a reference method. The reaction pathway was proposed.
Keywords: Kinetic determination; isoxsuprine HCl; (NBD-Cl); pharmaceutical preparations Kinetic determination; isoxsuprine HCl; (NBD-Cl); pharmaceutical preparations

Share and Cite

MDPI and ACS Style

El-Enany, N.; Belal, F.; Rizk, M. Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7- nitrobenzo-2-oxa-1,3-diazole (NBD-Cl). Sci. Pharm. 2006, 74, 99-119. https://doi.org/10.3797/scipharm.2006.74.99

AMA Style

El-Enany N, Belal F, Rizk M. Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7- nitrobenzo-2-oxa-1,3-diazole (NBD-Cl). Scientia Pharmaceutica. 2006; 74(3):99-119. https://doi.org/10.3797/scipharm.2006.74.99

Chicago/Turabian Style

El-Enany, N., F. Belal, and M. Rizk. 2006. "Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7- nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)" Scientia Pharmaceutica 74, no. 3: 99-119. https://doi.org/10.3797/scipharm.2006.74.99

APA Style

El-Enany, N., Belal, F., & Rizk, M. (2006). Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7- nitrobenzo-2-oxa-1,3-diazole (NBD-Cl). Scientia Pharmaceutica, 74(3), 99-119. https://doi.org/10.3797/scipharm.2006.74.99

Article Metrics

Back to TopTop