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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2015). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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Sci. Pharm. 2006, 74(3), 99-119; https://doi.org/10.3797/scipharm.2006.74.99 (registering DOI)

Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7- nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, 35516, Mansoura, Egypt
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Received: 5 January 2006 / Accepted: 22 March 2006 / Published: 30 September 2006
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Abstract

A simple and sensitive kinetic spectrophotometric method was developed for the determination of isoxsuprine in pharmaceutical preparations. The method is based upon a kinetic investigation of the coupling reaction between isoxsuprine HCl and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in borate buffer of pH 7.8 for a fixed time of 30 min. The absorbance of the yellow coloured product was measured at 395 nm. The absorbance- concentration plot was rectilinear over the range of 2-20 μg mL-1 (r= 0.9994) with minimum detectability of 0.6 μg mL-1 (1.75 x10-6M). The different experimental parameters affecting the development and stability of the colour were carefully studied and optimized. The determination of isoxsuprine by the fixed-concentration and rate-constant methods is also feasible with the calibration equations obtained, but the fixed time method has been found to be more applicable. The proposed method was further applied to the determination of the drug in formulations. The results obtained were in good agreement with those obtained using a reference method. The reaction pathway was proposed.
Keywords: Kinetic determination; isoxsuprine HCl; (NBD-Cl); pharmaceutical preparations Kinetic determination; isoxsuprine HCl; (NBD-Cl); pharmaceutical preparations
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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El-Enany, N.; Belal, F.; Rizk, M. Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7- nitrobenzo-2-oxa-1,3-diazole (NBD-Cl). Sci. Pharm. 2006, 74, 99-119.

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