NF-κB Activation Exacerbates, but Is not Required for Murine Bmpr2-Related Pulmonary Hypertension
Abstract
:1. Introduction
2. Experimental Section
2.1. Electrophoretic Mobility Shift Assay (EMSA)
2.2. IP Lipopolysaccharide (LPS)
2.3. Western Blotting
2.4. Quantitative RT-PCR
2.5. Luciferase Assays
2.6. Echocardiography and Hemodynamic Phenotyping
2.7. dnIkB Experiments
2.8. RelA Virus Administration
2.9. IKTA Mice
2.10. Morphometry
2.11. Statistical Methods
3. Results and Discussions
3.1. NF-κB Activation is Present in Human and Murine Pulmonary Hypertension
3.2. The BMP Pathway is Suppressed during Acute Inflammation
3.3. Expression of the Bmpr2R899X Transgene Suppresses Basal NF-κB Activation
3.4. NF-κB Activation Is not Required for Murine Bmpr2-Related PH
3.5. NF-κB Activation Exacerbates Murine Bmpr2-Related PH
3.5. Discussion
4. Conclusions
Acknowledgements
Author Contributions
Conflicts of Interest
References
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Talati, M.; Mutlak, H.; Lane, K.B.; Han, W.; Hemnes, A.; Mutlak, O.; Blackwell, T.; Zaynagetdinov, R.; Blackwell, T.S.; West, J. NF-κB Activation Exacerbates, but Is not Required for Murine Bmpr2-Related Pulmonary Hypertension. Diseases 2014, 2, 148-167. https://doi.org/10.3390/diseases2020148
Talati M, Mutlak H, Lane KB, Han W, Hemnes A, Mutlak O, Blackwell T, Zaynagetdinov R, Blackwell TS, West J. NF-κB Activation Exacerbates, but Is not Required for Murine Bmpr2-Related Pulmonary Hypertension. Diseases. 2014; 2(2):148-167. https://doi.org/10.3390/diseases2020148
Chicago/Turabian StyleTalati, Megha, Haitham Mutlak, Kirk B. Lane, Wei Han, Anna Hemnes, Outi Mutlak, Tom Blackwell, Rinat Zaynagetdinov, Timothy S. Blackwell, and James West. 2014. "NF-κB Activation Exacerbates, but Is not Required for Murine Bmpr2-Related Pulmonary Hypertension" Diseases 2, no. 2: 148-167. https://doi.org/10.3390/diseases2020148