Next Article in Journal
Wide-Angle Beam Scanning Leaky-Wave Antenna Using Spoof Surface Plasmon Polaritons Structure
Previous Article in Journal
New Nonlinear Second-Order Phase-Locked Loop with Adaptive Bandwidth Regulation
Article

Impedance Sensing Platform for Detection of the Food Pathogen Listeria monocytogenes

1
CNR NANOTEC Institute of Nanotechnology, via Monteroni, 73100 Lecce, Italy
2
DisTeBA, University of Salento, 73100 Lecce, Italy
3
Department of Mathematics and Physics, University of Salento, 73100 Lecce, Italy
4
CNR-ISPA, Istituto di Scienze delle Produzioni Alimentari-Consiglio Nazionale delle Ricerche, 73100 Lecce, Italy
*
Author to whom correspondence should be addressed.
Electronics 2018, 7(12), 347; https://doi.org/10.3390/electronics7120347
Received: 25 October 2018 / Revised: 17 November 2018 / Accepted: 19 November 2018 / Published: 23 November 2018
(This article belongs to the Section Bioelectronics)
A great improvement in food safety and quality controls worldwide has been achieved through the development of biosensing platforms. Foodborne pathogens continue to cause serious outbreaks, due to the ingestion of contaminated food. The development of new, sensitive, portable, high-throughput, and automated platforms is a primary objective to allow detection of pathogens and their toxins in foods. Listeria monocytogenes is one common foodborne pathogen. Major outbreaks of listeriosis have been caused by a variety of foods, including milk, soft cheeses, meat, fermented sausages, poultry, seafood and vegetable products. Due to its high sensitivity and easy setup, electrochemical impedance spectroscopy (EIS) has been extensively applied for biosensor fabrication and in particular in the field of microbiology as a mean to detect and quantify foodborne bacteria. Here we describe a miniaturized, portable EIS platform consisting of a microfluidic device with EIS sensors for the detection of L. monocytogenes in milk samples, connected to a portable impedance analyzer for on-field application in clinical and food diagnostics, but also for biosecurity purposes. To achieve this goal microelectrodes were functionalized with antibodies specific for L. monocytogenes. The binding and detection of L. monocytogenes was achieved in the range 2.2 × 103 cfu/mL to 1 × 102 with a Limit of Detection (LoD) of 5.5 cfu/mL. View Full-Text
Keywords: food pathogens; electrochemical impedance spectroscopy; surface activation food pathogens; electrochemical impedance spectroscopy; surface activation
Show Figures

Figure 1

MDPI and ACS Style

Chiriacò, M.S.; Parlangeli, I.; Sirsi, F.; Poltronieri, P.; Primiceri, E. Impedance Sensing Platform for Detection of the Food Pathogen Listeria monocytogenes. Electronics 2018, 7, 347. https://doi.org/10.3390/electronics7120347

AMA Style

Chiriacò MS, Parlangeli I, Sirsi F, Poltronieri P, Primiceri E. Impedance Sensing Platform for Detection of the Food Pathogen Listeria monocytogenes. Electronics. 2018; 7(12):347. https://doi.org/10.3390/electronics7120347

Chicago/Turabian Style

Chiriacò, Maria S., Ilaria Parlangeli, Fausto Sirsi, Palmiro Poltronieri, and Elisabetta Primiceri. 2018. "Impedance Sensing Platform for Detection of the Food Pathogen Listeria monocytogenes" Electronics 7, no. 12: 347. https://doi.org/10.3390/electronics7120347

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop