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Coatings 2019, 9(2), 134; https://doi.org/10.3390/coatings9020134

Argon Plasma Surface Modified Porcine Bone Substitute Improved Osteoblast-Like Cell Behavior

1
Department of Community Medicine, En Chu Kong Hospital, New Taipei City 237, Taiwan
2
Yuanpei University of Medical technology, Hsin Chu, Taipei 300, Taiwan
3
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan
4
Graduate Institute of Biomedical Materials & Tissue Engineering, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan
5
Dental Department, Taipei Medical University Hospital, Taipei 110, Taiwan
6
Department of General Dentistry, Chang Gung Memorial Hospital, Taipei 105, Taiwan
7
Graduate Institute of Dental & Craniofacial Science, Chang Gung University, Taoyuan 333, Taiwan
8
School of Dentistry, College of Medicine, China Medical University, Taichung 404, Taiwan
9
Dental Department, Taipei Medical University, Shuang-Ho hospital, Taipei 235, Taiwan
*
Authors to whom correspondence should be addressed.
Received: 14 December 2018 / Revised: 16 February 2019 / Accepted: 16 February 2019 / Published: 19 February 2019
(This article belongs to the Special Issue Surface Chemical Modification)
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Abstract

Low-temperature plasma-treated porcine grafts (PGPT) may be an effective means for treating demanding osseous defects and enhance our understanding of plasma-tissue engineering. We chemically characterized porcine grafts under low-temperature Argon plasma treatment (CAP) and evaluated their biocompatibility in-vitro. Our results showed that PGPT did not differ in roughness, dominant crystalline phases, absorption peaks corresponding to phosphate band peaks, or micro-meso pore size, compared to non-treated porcine grafts. The PGPT Ca/P ratio was 2.16; whereas the porcine control ratio was 2.04 (p < 0.05). PGPT’s [C 1s], [P 2p] and [Ca 2p] values were 24.3%, 5.6% and 11.0%, respectively, indicating that PGPT was an apatite without another crystalline phase. Cell viability and alkaline phosphatase assays revealed enhanced proliferation and osteoblastic differentiation for the cells cultivated in the PGPT media after 5 days (p < 0.05). The cells cultured in PGPT medium had higher bone sialoprotein and osteocalcin relative mRNA expression compared to cells cultured in non-treated porcine grafts (p < 0.05). CAP treatment of porcine particles did not modify the biomaterial’s surface and improved the proliferation and differentiation of osteoblast-like cells. View Full-Text
Keywords: argon plasma treatment; porcine bone graft; biological apatite; chemical properties; in-vitro behavior argon plasma treatment; porcine bone graft; biological apatite; chemical properties; in-vitro behavior
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Choy, C.S.; Salamanca, E.; Lin, P.Y.; Huang, H.-M.; Teng, N.-C.; Pan, Y.-H.; Chang, W.-J. Argon Plasma Surface Modified Porcine Bone Substitute Improved Osteoblast-Like Cell Behavior. Coatings 2019, 9, 134.

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