Next Article in Journal
Combined Effects of Sulfamethoxazole and Erythromycin on a Freshwater Microalga, Raphidocelis subcapitata: Toxicity and Oxidative Stress
Previous Article in Journal
Comparison of Antibiotic Resistance Profile of Escherichia coli between Pristine and Human-Impacted Sites in a River
Previous Article in Special Issue
Community-Acquired, Extended-Spectrum β-Lactamase-Producing and Extensively Drug-Resistant Escherichia coli in a 28-Year-Old Pyelonephritis Patient Lacking Risk Factors
Article

A New Twist: The Combination of Sulbactam/Avibactam Enhances Sulbactam Activity against Carbapenem-Resistant Acinetobacter baumannii (CRAB) Isolates

1
National/Regional Reference Laboratory for Antimicrobial Resistance (NRL), Servicio Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas, ANLIS “Dr. Carlos G. Malbrán”, C1282AFF Buenos Aires, Argentina
2
Center for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, CA 92831-3599, USA
3
Laboratorio de Bacteriología Clínica, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, C1120 Buenos Aires, Argentina
4
Research Service and GRECC, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH 44106, USA
5
Departments of Medicine, Pharmacology, Molecular Biology and Microbiology, Biochemistry, Proteomics and Bioinformatics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
6
CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology (Case VA CARES), Cleveland, OH 44106, USA
7
The Jackson Laboratory, Farmington, CT 06032, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Maria Lina Mezzatesta
Antibiotics 2021, 10(5), 577; https://doi.org/10.3390/antibiotics10050577
Received: 29 April 2021 / Revised: 10 May 2021 / Accepted: 11 May 2021 / Published: 13 May 2021
An increasing number of untreatable infections are recorded every year. Many studies have focused their efforts on developing new β-lactamase inhibitors to treat multi-drug resistant (MDR) isolates. In the present study, sulbactam/avibactam and sulbactam/relebactam combination were tested against 187 multi-drug resistant (MDR) Acinetobacter clinical isolates; both sulbactam/avibactam and sulbactam/relebactam restored sulbactam activity. A decrease ≥2 dilutions in sulbactam MICs was observed in 89% of the isolates when tested in combination with avibactam. Sulbactam/relebactam was able to restore sulbactam susceptibility in 40% of the isolates. In addition, the susceptibility testing using twenty-three A. baumannii AB5075 knockout strains revealed potential sulbactam and/or sulbactam/avibactam target genes. We observed that diazabicyclooctanes (DBOs) β-lactamase inhibitors combined with sulbactam restore sulbactam susceptibility against carbapenem-resistant Acinetobacter clinical isolates. However, relebactam was not as effective as avibactam when combined with sulbactam. Exploring novel combinations may offer new options to treat Acinetobacter spp. infections, especially for widespread oxacillinases and metallo-β-lactamases (MBLs) producers. View Full-Text
Keywords: Acinetobacter; carbapenem-resistance; sulbactam; avibactam; relebactam Acinetobacter; carbapenem-resistance; sulbactam; avibactam; relebactam
Show Figures

Figure 1

MDPI and ACS Style

Pasteran, F.; Cedano, J.; Baez, M.; Albornoz, E.; Rapoport, M.; Osteria, J.; Montaña, S.; Le, C.; Ra, G.; Bonomo, R.A.; Tolmasky, M.E.; Adams, M.; Corso, A.; Ramirez, M.S. A New Twist: The Combination of Sulbactam/Avibactam Enhances Sulbactam Activity against Carbapenem-Resistant Acinetobacter baumannii (CRAB) Isolates. Antibiotics 2021, 10, 577. https://doi.org/10.3390/antibiotics10050577

AMA Style

Pasteran F, Cedano J, Baez M, Albornoz E, Rapoport M, Osteria J, Montaña S, Le C, Ra G, Bonomo RA, Tolmasky ME, Adams M, Corso A, Ramirez MS. A New Twist: The Combination of Sulbactam/Avibactam Enhances Sulbactam Activity against Carbapenem-Resistant Acinetobacter baumannii (CRAB) Isolates. Antibiotics. 2021; 10(5):577. https://doi.org/10.3390/antibiotics10050577

Chicago/Turabian Style

Pasteran, Fernando, Jose Cedano, Michelle Baez, Ezequiel Albornoz, Melina Rapoport, Jose Osteria, Sabrina Montaña, Casin Le, Grace Ra, Robert A. Bonomo, Marcelo E. Tolmasky, Mark Adams, Alejandra Corso, and Maria S. Ramirez 2021. "A New Twist: The Combination of Sulbactam/Avibactam Enhances Sulbactam Activity against Carbapenem-Resistant Acinetobacter baumannii (CRAB) Isolates" Antibiotics 10, no. 5: 577. https://doi.org/10.3390/antibiotics10050577

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop