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Article

Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece

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Faculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece
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Leibniz Institute of Photonic Technology (IPHT), 07745 Jena, Germany
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InfectoGnostics Research Campus, 07743 Jena, Germany
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Faculty of Animal Science, University of Thessaly, 41110 Larissa, Greece
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Institut fuer Medizinische Mikrobiologie und Hygiene, Medizinische Fakultaet “Carl Gustav Carus”, TU Dresden, 01307 Dresden, Germany
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Institute of Physical Chemistry, Friedrich Schiller University Jena, 07737 Jena, Germany
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Faculty of Medicine, University of Thessaly, 41500 Larissa, Greece
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Faculty of Public and Integrated Health, University of Thessaly, 43100 Karditsa, Greece
*
Author to whom correspondence should be addressed.
Academic Editor: Marc Maresca
Antibiotics 2021, 10(4), 389; https://doi.org/10.3390/antibiotics10040389
Received: 24 February 2021 / Revised: 1 April 2021 / Accepted: 2 April 2021 / Published: 4 April 2021
The prevalence of multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Enterobacteriaceae is increasing worldwide. The present study aimed to provide an overview of the multidrug resistance phenotype and genotype of ESBL-producing Escherichia coli (E. coli) isolates of livestock and wild bird origin in Greece. Nineteen phenotypically confirmed ESBL-producing E. coli strains isolated from fecal samples of cattle (n = 7), pigs (n = 11) and a Eurasian magpie that presented resistance to at least one class of non β-lactam antibiotics, were selected and genotypically characterized. A DNA-microarray based assay was used, which allows the detection of various genes associated with antimicrobial resistance. All isolates harbored blaCTX-M-1/15, while blaTEM was co-detected in 13 of them. The AmpC gene blaMIR was additionally detected in one strain. Resistance genes were also reported for aminoglycosides in all 19 isolates, for quinolones in 6, for sulfonamides in 17, for trimethoprim in 14, and for macrolides in 8. The intI1 and/or tnpISEcp1 genes, associated with mobile genetic elements, were identified in all but two isolates. This report describes the first detection of multidrug resistance genes among ESBL-producing E. coli strains retrieved from feces of cattle, pigs, and a wild bird in Greece, underlining their dissemination in diverse ecosystems and emphasizing the need for a One-Health approach when addressing the issue of antimicrobial resistance. View Full-Text
Keywords: ESBL; Escherichia coli; multidrug resistance; antimicrobial resistance genes; cattle; pigs; Eurasian magpie; Greece ESBL; Escherichia coli; multidrug resistance; antimicrobial resistance genes; cattle; pigs; Eurasian magpie; Greece
MDPI and ACS Style

Athanasakopoulou, Z.; Reinicke, M.; Diezel, C.; Sofia, M.; Chatzopoulos, D.C.; Braun, S.D.; Reissig, A.; Spyrou, V.; Monecke, S.; Ehricht, R.; Tsilipounidaki, K.; Giannakopoulos, A.; Petinaki, E.; Billinis, C. Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece. Antibiotics 2021, 10, 389. https://doi.org/10.3390/antibiotics10040389

AMA Style

Athanasakopoulou Z, Reinicke M, Diezel C, Sofia M, Chatzopoulos DC, Braun SD, Reissig A, Spyrou V, Monecke S, Ehricht R, Tsilipounidaki K, Giannakopoulos A, Petinaki E, Billinis C. Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece. Antibiotics. 2021; 10(4):389. https://doi.org/10.3390/antibiotics10040389

Chicago/Turabian Style

Athanasakopoulou, Zoi, Martin Reinicke, Celia Diezel, Marina Sofia, Dimitris C. Chatzopoulos, Sascha D. Braun, Annett Reissig, Vassiliki Spyrou, Stefan Monecke, Ralf Ehricht, Katerina Tsilipounidaki, Alexios Giannakopoulos, Efthymia Petinaki, and Charalambos Billinis. 2021. "Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece" Antibiotics 10, no. 4: 389. https://doi.org/10.3390/antibiotics10040389

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