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Genetic Features of Extended-Spectrum β-Lactamase-Producing Escherichia coli from Poultry in Mayabeque Province, Cuba

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National Center for Animal and Plant Health, Tapaste Road and National Highway, Postal Mail 10, San José de las Lajas, Mayabeque 32700, Cuba
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Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, 3012 Bern, Switzerland
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Mayabeque Poultry Company, Ave 69, # 7210 between 72 and 74 Guines, Mayabeque 33900, Cuba
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Center for Nuclear in Agriculture, University of São Paulo, Piracicaba, SP 13400-970, Brazil
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School of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada
*
Author to whom correspondence should be addressed.
Antibiotics 2021, 10(2), 107; https://doi.org/10.3390/antibiotics10020107
Received: 4 December 2020 / Revised: 13 January 2021 / Accepted: 17 January 2021 / Published: 22 January 2021
(This article belongs to the Section Mechanism and Evolution of Antibiotic Resistance)
A total of 434 poultry cloacal samples were collected from seven different farms in different years (2013–2015) in the Cuban province of Mayabeque and analyzed for the presence of third-generation cephalosporin-resistant Escherichia coli (3GC-R-Ec). Sixty-two 3GC-R-Ec isolates were recovered in total from the farms, with detection rates of 2.9% in 2013, 10.3% in 2014, and 28.7% in 2015. Characterization of 32 3GC-R-Ec isolates revealed the presence of the extended-spectrum β-lactamase (ESBL) genes blaCTX-M-1 (n = 27), blaCTX-M-15 (n = 4), and blaCTX-M-1 together with blaLAP-2 (n = 1). The isolates also contained different proportions of genes conferring decreased susceptibility to sulfonamides (sul1, sul2, sul3), trimethoprim (dfrA1, dfrA7, dfrA12, dfrA14, dfrA17), tetracyclines (tet(A), tet(B)), aminoglycosides (aac(6′)-Ib-cr, strA, strB), chloramphenicol (cmlA1, floR), macrolides (mph(A), mph(D)), and quinolones (qnrS, qnrB, aac(6′)-Ib-cr) as well as mutations in the fluoroquinolone-resistance determining regions of GyrA (S83L, D87N, D87Y) and ParC (S80I, E84G). The isolates belonged to 23 different sequence types and to phylogroups A (n = 25), B1 (n = 5), and D (n = 2), and they contained plasmid-associated incompatibility groups FII, X1, HI1, HI2, N, FIA, and FIB. These findings reveal a genetically diverse population of multiresistant ESBL-producing E. coli in poultry farms in Cuba, which suggests multiple sources of contamination and the acquisition of antibiotic resistance genes. View Full-Text
Keywords: third-generation cephalosporins; ESBL; Escherichia coli; chicken; antibiotic resistance; genotyping third-generation cephalosporins; ESBL; Escherichia coli; chicken; antibiotic resistance; genotyping
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MDPI and ACS Style

Baez, M.; Espinosa, I.; Collaud, A.; Miranda, I.; Montano, D.d.l.N.; Feria, A.L.; Hernández-Fillor, R.E.; Obregón, D.; Alfonso, P.; Perreten, V. Genetic Features of Extended-Spectrum β-Lactamase-Producing Escherichia coli from Poultry in Mayabeque Province, Cuba. Antibiotics 2021, 10, 107. https://doi.org/10.3390/antibiotics10020107

AMA Style

Baez M, Espinosa I, Collaud A, Miranda I, Montano DdlN, Feria AL, Hernández-Fillor RE, Obregón D, Alfonso P, Perreten V. Genetic Features of Extended-Spectrum β-Lactamase-Producing Escherichia coli from Poultry in Mayabeque Province, Cuba. Antibiotics. 2021; 10(2):107. https://doi.org/10.3390/antibiotics10020107

Chicago/Turabian Style

Baez, Michel, Ivette Espinosa, Alexandra Collaud, Iliana Miranda, Damarys d.l.N. Montano, Angel L. Feria, Rosa E. Hernández-Fillor, Dasiel Obregón, Pastor Alfonso, and Vincent Perreten. 2021. "Genetic Features of Extended-Spectrum β-Lactamase-Producing Escherichia coli from Poultry in Mayabeque Province, Cuba" Antibiotics 10, no. 2: 107. https://doi.org/10.3390/antibiotics10020107

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