Antibacterial Activity of LCB10-0200 against Klebsiella pneumoniae
Abstract
:1. Introduction
2. Results
2.1. In Vitro Activity of LCB10-0200 against K. pneumoniae
2.2. In Vivo Activity of LCB10-0200 against K. pneumoniae
3. Discussion
4. Materials and Methods
4.1. Antimicrobial Agents and Bacterial Strains
4.2. Minimum Inhibition Concentration (MIC) Determination
4.3. Systemic Infection Mouse Model
4.4. Thigh Infection Mouse Model
4.5. Urinary Tract Infection Mouse Model
4.6. Animal Ethical Approval
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Bacterial Strains | Compound MIC (mg/L) | ||
---|---|---|---|
LCB10-0200 | SID | ATC | |
K. pneumoniae ATCC 13883 | 0.125 | >64 | 16 |
K. pneumoniae ATCC700603 | 2 | >64 | 64 |
K. pneumoniae 3835 | 1 | >64 | 64 |
Strain | Beta-Lactamase | MIC (mg/L) | ||
---|---|---|---|---|
LCB10-0200 | Ceftazidime | Ciprofloxacin | ||
K. pneumoniae 49 | KPC-2 | 8 | >32 | >32 |
K. pneumoniae 50 | KPC-2 | 8 | >32 | >32 |
K. pneumoniae 51 | KPC-2 | 4 | >32 | >32 |
K. pneumoniae 52 | GES-5 | 1 | >32 | 2 |
K. pneumoniae 53 | NDM-1 | >32 | >32 | >32 |
K. pneumoniae 54 | OXA-232 | 2 | >32 | >32 |
K. pneumoniae 55 | OXA-232 | 32 | >32 | >32 |
Microorganism Inoculum (CFU/mouse) a | Antimicrobial Agents b | MIC (mg/L) | ED50 (mg/kg) (95% Confidence Limits) |
---|---|---|---|
K. pneumoniae ATCC13883 | LCB10-0200 | 0.125 | <0.8 |
(5 × 107) | Ceftazidime | 0.125 | <0.8 |
K. pneumoniae ATCC700603 | LCB10-0200 | 0.5 | <2.5 |
(5 × 107) | Ceftazidime | 8 | >20 |
K. pneumoniae 3835 | LCB10-0200 | 1 | 25.01 (11.37–50.01) |
(2 × 108) | Ceftazidime | 64 | >40 |
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Oh, S.-H.; Kim, Y.-R.; Park, H.-S.; Oh, K.-M.; Cho, Y.-L.; Kwak, J.-H. Antibacterial Activity of LCB10-0200 against Klebsiella pneumoniae. Antibiotics 2021, 10, 1185. https://doi.org/10.3390/antibiotics10101185
Oh S-H, Kim Y-R, Park H-S, Oh K-M, Cho Y-L, Kwak J-H. Antibacterial Activity of LCB10-0200 against Klebsiella pneumoniae. Antibiotics. 2021; 10(10):1185. https://doi.org/10.3390/antibiotics10101185
Chicago/Turabian StyleOh, Sang-Hun, Young-Rok Kim, Hee-Soo Park, Kyu-Man Oh, Young-Lag Cho, and Jin-Hwan Kwak. 2021. "Antibacterial Activity of LCB10-0200 against Klebsiella pneumoniae" Antibiotics 10, no. 10: 1185. https://doi.org/10.3390/antibiotics10101185