Characterization of Fitness Cost Caused by Tigecycline-Resistance Gene tet(X6) in Different Host Bacteria
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains and Plasmids
2.2. Quantitative Real-Time PCR
2.3. Effects of tet(X6) Overexpression on Bacterial Growth
2.4. In Vitro Competition and Biofilm Formation
2.5. Galleria mellonella Infection Model
2.6. Statistical Analysis
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Genes | Sequences (5′ to 3′) | Amplicon Size (bp) |
---|---|---|
tet(X6) | F: CGAGCTCATGACTTTACTAAAACATAAAAAAATTAC R: CCCAAGCTTTTATAGATTCATTAGTTTTTGGAAAGAA | 1149 |
qPCR-tet(X6) | F: TGTCGTTGATTTTCTCCTG R: TTGATTCTGCCTGTGCTT | 332 |
16S rRNA | F: TTCGGGAACCGTGAGA R: CTGGCAACAAAGGATAAGG | 103 |
Strains | MIC (μg/mL) | MIC (μg/mL) with l-Arabinose (10 mM) |
---|---|---|
E. coli TOP10 (tet(X6)/pBAD) | 0.125 | 1 |
S. Enteritidis ATCC13076 (tet(X6)/pBAD) | 1 | 8 |
P. mirabilis HS1-T (tet(X6)/pBAD) | 4 | 8 |
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Jiang, L.; Cai, W.; Tang, F.; Wang, Z.; Liu, Y. Characterization of Fitness Cost Caused by Tigecycline-Resistance Gene tet(X6) in Different Host Bacteria. Antibiotics 2021, 10, 1172. https://doi.org/10.3390/antibiotics10101172
Jiang L, Cai W, Tang F, Wang Z, Liu Y. Characterization of Fitness Cost Caused by Tigecycline-Resistance Gene tet(X6) in Different Host Bacteria. Antibiotics. 2021; 10(10):1172. https://doi.org/10.3390/antibiotics10101172
Chicago/Turabian StyleJiang, Lijie, Wenhui Cai, Feifei Tang, Zhiqiang Wang, and Yuan Liu. 2021. "Characterization of Fitness Cost Caused by Tigecycline-Resistance Gene tet(X6) in Different Host Bacteria" Antibiotics 10, no. 10: 1172. https://doi.org/10.3390/antibiotics10101172