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Review

Current Status of Endolysin-Based Treatments against Gram-Negative Bacteria

Departamento de Genética, Evolução, Microbiologia e Imunologia, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Rua Monteiro Lobato 255, Campinas 13083-862, Brazil
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Academic Editor: Hongping Wei
Antibiotics 2021, 10(10), 1143; https://doi.org/10.3390/antibiotics10101143
Received: 4 June 2021 / Revised: 14 July 2021 / Accepted: 21 July 2021 / Published: 22 September 2021
(This article belongs to the Special Issue Bacteriophage Lysins in the Era of Antibiotic Resistance)
The prevalence of multidrug-resistant Gram-negative bacteria is a public health concern. Bacteriophages and bacteriophage-derived lytic enzymes have been studied in response to the emergence of multidrug-resistant bacteria. The availability of tRNAs and endolysin toxicity during recombinant protein expression is circumvented by codon optimization and lower expression levels using inducible pET-type plasmids and controlled cultivation conditions, respectively. The use of polyhistidine tags facilitates endolysin purification and alters antimicrobial activity. Outer membrane permeabilizers, such as organic acids, act synergistically with endolysins, but some endolysins permeate the outer membrane of Gram-negative bacteria per se. However, the outer membrane permeation mechanisms of endolysins remain unclear. Other strategies, such as the co-administration of endolysins with polymyxins, silver nanoparticles, and liposomes confer additional outer membrane permeation. Engineered endolysins comprising domains for outer membrane permeation is also a strategy used to overcome the current challenges on the control of multidrug-resistant Gram-negative bacteria. Metagenomics is a new strategy for screening endolysins with interesting antimicrobial properties from uncultured phage genomes. Here, we review the current state of the art on the heterologous expression of endolysin, showing the potential of bacteriophage endolysins in controlling bacterial infections. View Full-Text
Keywords: nosocomial pathogen; bacteriophage; enzybiotic; peptide tag; bactericidal activity; biocontrol; antibiotic substitute nosocomial pathogen; bacteriophage; enzybiotic; peptide tag; bactericidal activity; biocontrol; antibiotic substitute
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MDPI and ACS Style

Gontijo, M.T.P.; Jorge, G.P.; Brocchi, M. Current Status of Endolysin-Based Treatments against Gram-Negative Bacteria. Antibiotics 2021, 10, 1143. https://doi.org/10.3390/antibiotics10101143

AMA Style

Gontijo MTP, Jorge GP, Brocchi M. Current Status of Endolysin-Based Treatments against Gram-Negative Bacteria. Antibiotics. 2021; 10(10):1143. https://doi.org/10.3390/antibiotics10101143

Chicago/Turabian Style

Gontijo, Marco T.P., Genesy P. Jorge, and Marcelo Brocchi. 2021. "Current Status of Endolysin-Based Treatments against Gram-Negative Bacteria" Antibiotics 10, no. 10: 1143. https://doi.org/10.3390/antibiotics10101143

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