Next Article in Journal / Special Issue
Droplet Velocity Measurement Based on Dielectric Layer Thickness Variation Using Digital Microfluidic Devices
Previous Article in Journal
Continuous Glucose Monitoring in Resource-Constrained Settings for Hypoglycaemia Detection: Looking at the Problem from the Other Side of the Coin
Previous Article in Special Issue
S-Layer Protein-Based Biosensors
Article Menu
Issue 2 (June) cover image

Export Article

Open AccessFeature PaperArticle
Biosensors 2018, 8(2), 44;

Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask

Laboratory for Sensors, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Köhler Allee 103, D-79110 Freiburg, Germany
Jobst Technologies GmbH, Engesserstraße 4b, D-79108 Freiburg, Germany
Department of Physics, University of Oslo, 1048 Blindern, N-0316 Oslo, Norway
Author to whom correspondence should be addressed.
Received: 16 March 2018 / Revised: 17 April 2018 / Accepted: 23 April 2018 / Published: 26 April 2018
(This article belongs to the Special Issue Micro and Nanoscale Biosensors)
Full-Text   |   PDF [7715 KB, uploaded 2 May 2018]   |  


The Sensing Cell Culture Flask (SCCF) is a cell culture monitoring system accessing the cellular microenvironment in 2D cell culture using electrochemical microsensors. The system is based on microfabricated sensor chips embedded in standard cell culture flasks. Ideally, the sensor chips could be equipped with any electrochemical sensor. Its transparency allows optical inspection of the cells during measurement. The surface of the sensor chip is in-plane with the flask surface allowing undisturbed cell growth on the sensor chip. A custom developed rack system allows easy usage of multiple flasks in parallel within an incubator. The presented data demonstrates the application of the SCCF with brain tumor (T98G) and breast cancer (T-47D) cells. Amperometric oxygen sensors were used to monitor cellular respiration with different incubation conditions. Cellular acidification was accessed with potentiometric pH sensors using electrodeposited iridium oxide films. The system itself provides the foundation for electrochemical monitoring systems in 3D cell culture. View Full-Text
Keywords: cell culture monitoring; microsensor; oxygen; pH; hypoxia cell culture monitoring; microsensor; oxygen; pH; hypoxia

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Kieninger, J.; Tamari, Y.; Enderle, B.; Jobst, G.; Sandvik, J.A.; Pettersen, E.O.; Urban, G.A. Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask. Biosensors 2018, 8, 44.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top