Electrochemical Impedance Immunoassay for ALS-Associated Neurofilament Protein: Matrix Effect on the Immunoplatform
Abstract
:1. Introduction
2. Experimental
2.1. Materials
2.2. Preparation of Immunoplatform
2.3. Nf-L Detection by EIS
3. Results and Discussion
3.1. Preparation of Immunoplatform
3.2. Nf-L Detection in Buffer and Serum Media
3.3. Target Nf-L Determination in ALS Patients’ Serum
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample ID | Gender | Age | RCT (kΩ∙cm2) | Nf-L (ng/mL) Serum | Nf-L (ng/mL) Buffer |
---|---|---|---|---|---|
1 | Male | 40 s | 4.1 ± 0.2 | 4.4 ± 0.7 | 6.0 ± 0.9 |
2 | 50 s | 5.1 ± 0.4 | 8.9 ± 1.6 | 12 ± 2.0 | |
3 | 60 s | 4.8 ± 0.5 | 7.4 ± 2.2 | 9.7 ± 2.7 | |
4 | 70 s | 5.1 ± 0.4 | 8.9 ± 1.8 | 12 ± 2.3 | |
5 | 80 s | 5.5 ± 0.3 | 10.3 ± 1.3 | 13 ± 1.6 | |
6 | Female | 30 s | 3.9 ± 0.2 | 3.9 ± 1.0 | 5.3 ± 1.2 |
7 | 50 s | 4.2 ± 0.3 | 5.2 ± 1.1 | 7.0 ± 1.4 | |
8 | 60 s | 4.1 ± 0.2 | 4.7 ± 0.8 | 6.3 ± 0.9 | |
9 | 60 s | 4.3 ± 0.4 | 5.5 ± 1.8 | 7.3 ± 2.2 | |
10 | 70 s | 4.9 ± 0.2 | 7.9 ± 0.8 | 10 ± 1.0 |
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Adil, O.; Shamsi, M.H. Electrochemical Impedance Immunoassay for ALS-Associated Neurofilament Protein: Matrix Effect on the Immunoplatform. Biosensors 2023, 13, 247. https://doi.org/10.3390/bios13020247
Adil O, Shamsi MH. Electrochemical Impedance Immunoassay for ALS-Associated Neurofilament Protein: Matrix Effect on the Immunoplatform. Biosensors. 2023; 13(2):247. https://doi.org/10.3390/bios13020247
Chicago/Turabian StyleAdil, Omair, and Mohtashim H. Shamsi. 2023. "Electrochemical Impedance Immunoassay for ALS-Associated Neurofilament Protein: Matrix Effect on the Immunoplatform" Biosensors 13, no. 2: 247. https://doi.org/10.3390/bios13020247