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Sensing Hydration of Biomimetic Cell Membranes

Faculty of Materials Engineering and Technical Physics, Institute of Physics, Division of Molecular Physics, Poznan University of Technology, Piotrowo 3, 60-965 Poznan, Poland
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Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Biosensors 2021, 11(7), 241; https://doi.org/10.3390/bios11070241
Received: 31 May 2021 / Revised: 6 July 2021 / Accepted: 6 July 2021 / Published: 16 July 2021
Biological membranes play a vital role in cell functioning, providing structural integrity, controlling signal transduction, and controlling the transport of various chemical species. Owing to the complex nature of biomembranes, the self-assembly of lipids in aqueous media has been utilized to develop model systems mimicking the lipid bilayer structure, paving the way to elucidate the mechanisms underlying various biological processes, as well as to develop a number of biomedical and technical applications. The hydration properties of lipid bilayers are crucial for their activity in various cellular processes. Of particular interest is the local membrane dehydration, which occurs in membrane fusion events, including neurotransmission, fertilization, and viral entry. The lack of universal technique to evaluate the local hydration state of the membrane components hampers understanding of the molecular-level mechanisms of these processes. Here, we present a new approach to quantify the hydration state of lipid bilayers. It takes advantage of the change in the lateral diffusion of lipids that depends on the number of water molecules hydrating them. Using fluorescence recovery after photobleaching technique, we applied this approach to planar single and multicomponent supported lipid bilayers. The method enables the determination of the hydration level of a biomimetic membrane down to a few water molecules per lipid. View Full-Text
Keywords: hydration; lipid mobility; lateral diffusion; FRAP; model biomembrane; solid supported lipid bilayer; biological water; hydration sensing hydration; lipid mobility; lateral diffusion; FRAP; model biomembrane; solid supported lipid bilayer; biological water; hydration sensing
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MDPI and ACS Style

Chattopadhyay, M.; Orlikowska, H.; Krok, E.; Piatkowski, L. Sensing Hydration of Biomimetic Cell Membranes. Biosensors 2021, 11, 241. https://doi.org/10.3390/bios11070241

AMA Style

Chattopadhyay M, Orlikowska H, Krok E, Piatkowski L. Sensing Hydration of Biomimetic Cell Membranes. Biosensors. 2021; 11(7):241. https://doi.org/10.3390/bios11070241

Chicago/Turabian Style

Chattopadhyay, Madhurima, Hanna Orlikowska, Emilia Krok, and Lukasz Piatkowski. 2021. "Sensing Hydration of Biomimetic Cell Membranes" Biosensors 11, no. 7: 241. https://doi.org/10.3390/bios11070241

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