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Article

Paper-Based Airborne Bacteria Collection and DNA Extraction Kit

1
Department of Mechanical Engineering and Applied Mechanics, School of Engineering and Applied Science, University of Pennsylvania, 216 Towne Building, 220 S. 33rd Street, Philadelphia, PA 19104, USA
2
Department of Chemistry, Hanyang University, Seoul 04763, Korea
3
Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology (GIST), 123 Cheomdan-gwagiro, Gwangju 61005, Korea
*
Author to whom correspondence should be addressed.
Biosensors 2021, 11(10), 375; https://doi.org/10.3390/bios11100375
Received: 15 September 2021 / Revised: 1 October 2021 / Accepted: 4 October 2021 / Published: 7 October 2021
(This article belongs to the Special Issue 3D Printing for Point-of-Care In Vitro Diagnostic Devices)
The critical risk from airborne infectious diseases, bio-weapons, and harmful bacteria is currently the highest it has ever been in human history. The requirement for monitoring airborne pathogens has gradually increased to defend against bioterrorism or prevent pandemics, especially via simple and low-cost platforms which can be applied in resource-limited settings. Here, we developed a paper-based airborne bacteria collection and DNA extraction kit suitable for simple application with minimal instruments. Airborne sample collection and DNA extraction for PCR analysis were integrated in the paper kit. We created an easy-to-use paper-based air monitoring system using 3D printing technology combined with an air pump. The operation time of the entire process, comprising air sampling, bacterial cell lysis, purification and concentration of DNA, and elution of the DNA analyte, was within 20 min. All the investigations and optimum settings were tested in a custom-designed closed cabinet system. In the fabricated cabinet system, the paper kit operated effectively at a temperature of 25–35 °C and 30–70% relative humidity for air containing 10–106 CFU Staphylococcus aureus. This paper kit could be applied for simple, rapid, and cost-effective airborne pathogen monitoring. View Full-Text
Keywords: airborne pathogens; DNA extraction; bacteria detection; environment; air monitoring; lateral flow strip; bio-aerosol airborne pathogens; DNA extraction; bacteria detection; environment; air monitoring; lateral flow strip; bio-aerosol
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MDPI and ACS Style

Seok, Y.; Lee, J.; Kim, M.-G. Paper-Based Airborne Bacteria Collection and DNA Extraction Kit. Biosensors 2021, 11, 375. https://doi.org/10.3390/bios11100375

AMA Style

Seok Y, Lee J, Kim M-G. Paper-Based Airborne Bacteria Collection and DNA Extraction Kit. Biosensors. 2021; 11(10):375. https://doi.org/10.3390/bios11100375

Chicago/Turabian Style

Seok, Youngung, Joonseok Lee, and Min-Gon Kim. 2021. "Paper-Based Airborne Bacteria Collection and DNA Extraction Kit" Biosensors 11, no. 10: 375. https://doi.org/10.3390/bios11100375

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