Biocompatibility of Cyclopropylamine-Based Plasma Polymers Deposited at Sub-Atmospheric Pressure on Poly (ε-caprolactone) Nanofiber Meshes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fabrication of NFs and Plasma Polymer Deposition
2.2. XPS Analysis
2.3. SEM Imaging
2.4. Cell Culturing
2.5. Live/dead Assay by Fluorescence Imaging
2.6. MTT Assay
2.7. Examination of Cell Morphology by SEM
3. Results and Discussion
3.1. Surface Morphology
3.2. Surface Chemical Composition
3.3. Cell Adhesion and Proliferation Studies
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Deposition Duration (s) | Before TFBA Derivatization | Incorporated Amount of F after TFBA Derivatization | ||
---|---|---|---|---|
C (%) | O (%) | N (%) | F (%) | |
0 | 76.3 ± 0.4 | 23.7 ± 0.4 | 0 | 0 |
10 | 79.0 ± 1.0 | 8.9 ± 0.2 | 12.1 ± 0.9 | 4.2 ± 0.4 |
20 | 80.2 ± 0.9 | 9.3 ± 0.9 | 10.5 ± 1.5 | 5.3 ± 0.4 |
60 | 76.3 ± 0.9 | 4.6 ± 0.4 | 19.1 ± 1.1 | 5.8 ± 0.5 |
90 | 76.3 ± 1.5 | 3.1 ± 0.6 | 20.6 ± 1.0 | 4.5 ± 1.2 |
120 | 75.9 ± 0.8 | 3.1 ± 1.1 | 21.0 ± 0.6 | 4.6 ± 0.5 |
240 | 78.5 ± 1.1 | 2.2 ± 0.6 | 19.3 ± 1.0 | 3.6 ± 0.8 |
360 | 81.1 ± 1.1 | 1.5 ± 0.5 | 17.4 ± 0.9 | 3.4 ± 0.4 |
Deposition Duration (s) | C–C/C–H [C1] 285.0 eV | C–NHx [C2] 285.9 eV | C=N/C≡N/C–O [C3] 286.5 eV | N–C=O/C=O [C4] 288.0 eV | O–C=O [C5] 289.0 eV |
---|---|---|---|---|---|
0 | 58.9 ± 0.9 | - | 25.8 ± 1.0 | - | 15.3 ± 0.4 |
10 | 54.3 ± 1.7 | 7.3 ± 1.1 | 27.5 ± 0.9 | 6.0 ± 0.4 | 5.0 ± 0.3 |
20 | 51.2 ± 1.5 | 9.0 ± 1.1 | 29.3 ± 1.5 | 6.4 ± 0.7 | 5.2 ± 0.5 |
60 | 48.6 ± 1.6 | 6.3 ± 1.2 | 34.3 ± 0.6 | 8.2 ± 0.7 | 2.7 ± 0.2 |
90 | 47.0 ± 1.6 | 5.8 ± 1.3 | 36.8 ± 2.2 | 7.9 ± 1.2 | 2.6 ± 0.3 |
120 | 49.4 ± 1.6 | 5.2 ± 0.9 | 34.6 ± 1.8 | 7.9 ± 0.3 | 2.8 ± 0.3 |
240 | 55.7 ± 1.9 | 6.7 ± 1.7 | 31.0 ± 3.6 | 5.3 ± 0.5 | 1.4 ± 0.3 |
360 | 61.2 ± 1.8 | 6.4 ± 0.6 | 26.2 ± 2.5 | 5.1 ± 0.5 | 1.1 ± 0.6 |
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Chan, K.V.; Asadian, M.; Onyshchenko, I.; Declercq, H.; Morent, R.; De Geyter, N. Biocompatibility of Cyclopropylamine-Based Plasma Polymers Deposited at Sub-Atmospheric Pressure on Poly (ε-caprolactone) Nanofiber Meshes. Nanomaterials 2019, 9, 1215. https://doi.org/10.3390/nano9091215
Chan KV, Asadian M, Onyshchenko I, Declercq H, Morent R, De Geyter N. Biocompatibility of Cyclopropylamine-Based Plasma Polymers Deposited at Sub-Atmospheric Pressure on Poly (ε-caprolactone) Nanofiber Meshes. Nanomaterials. 2019; 9(9):1215. https://doi.org/10.3390/nano9091215
Chicago/Turabian StyleChan, Ke Vin, Mahtab Asadian, Iuliia Onyshchenko, Heidi Declercq, Rino Morent, and Nathalie De Geyter. 2019. "Biocompatibility of Cyclopropylamine-Based Plasma Polymers Deposited at Sub-Atmospheric Pressure on Poly (ε-caprolactone) Nanofiber Meshes" Nanomaterials 9, no. 9: 1215. https://doi.org/10.3390/nano9091215