Anchoring Property of a Novel Hydrophilic Lipopolymer, HDAS-SHP, Post-Inserted in Preformed Liposomes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis of HDAS-Lipopolymers
2.2. Critical Micellar Concentration (CMC)
2.3. Liposome Preparation
2.4. Post-Insertion of Lipopolymers in Preformed Liposomes
2.5. Phospholipid Concentration
2.6. Dynamic Light Scattering
2.7. Anchoring Property of the Lipopolymers
2.8. Stability of Surface-Modified Liposomes in Serum
2.9. Complement Activation by HDAS-SHP- and DSPE-PEG2000-Liposomes
2.10. Complement Activation by HDAS-SHP- and DSPE-PEG2000-Liposomes in Mice
2.11. Data Analysis
3. Results
3.1. CMC of HDAS-Lipopolymers and Characteristics of Resultant Micelles
3.2. Post-Insertion of HDAS-Lipopolymers and Their Retention in Liposome Bilayer
3.3. Liposome Stability in Serum and Effect of Liposomes on Complement Pathway
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Appendix A
Preparation of Fluorescein isothiocyanate-Loaded Liposomes
Post-Insertion of HDAS-SHP and DSPE-PEG2000
Samples | Size, nm | Polydispersity | ζ-Potential, mV | [FITC], µg/mL |
---|---|---|---|---|
Plain Liposomes | 162 ± 1 | 0.05 ± 0.01 | −55.7 ± 2.7 | 1.41 ± 0.19 |
DSPE-PEG2000 Liposomes | 172 ± 2 | 0.081 ± 0.03 | −68.0 ± 1.1 | 0.81 ± 0.06 |
HDAS-SHP Liposomes | 174 ± 1 | 0.050 ± 0.02 | −59.9 ± 3.1 | 0.91 ± 0.12 |
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Micelles | Size (nm) | Polydispersity | ζ-Potential (mV) |
---|---|---|---|
DSPE-PEG2000 | 29 ± 5 | 0.069 ± 0.02 | −34.4 ± 2.3 |
HDAS-PEG2000 | 71 ± 2 * | 0.073 ± 0.03 | −3.7 ± 0.8 * |
HDAS-SHP | 53 ± 4 *,# | 0.063 ± 0.01 | +28.4 ± 0.5 *,# |
Liposomes | Size (nm) | Polydispersity | ζ-Potential (mV) | |||
---|---|---|---|---|---|---|
t0 | t1 | t0 | t1 | t0 | t1 | |
Plain | 120 ± 2 | - | 0.092 ± 0.02 | - | −12.8 ± 1.2 | - |
DSPE-PEG2000 | 181 ± 2 * | 144 ± 1 * | 0.081 ± 0.03 | 0.038 ± 0.02 | −39.6 ± 3.1 | −47.3 ± 3.7 |
HDAS-PEG2000 | 169 ± 2 *,# | 132 ± 2 *,# | 0.042 ± 0.02 | 0.038 ± 0.01 | −9.1 ± 0.71 | −8.7 ± 0.9 |
HDAS-SHP | 161 ± 3 *,# | 135 ± 2 *,# | 0.076 ± 0.03 | 0.038 ± 0.01 | +39.1 ± 3.1 | +33.5 ± 2.6 |
Samples | Time (h) | kDISORP | |||
---|---|---|---|---|---|
0 | 6 | 12 | 24 | (%/h) | |
DSPE-PEG2000 | 100 | 98.04 ± 0.4 | 90.60 ± 0.7 | 89.84 ± 1.1 | −0.45 |
HDAS-PEG2000 | 100 | 98.11 ± 0.3 | 94.99 ± 0.5 | 84.39 ± 0.9 | −0.67 |
HDAS-SHP | 100 | 92.79 ± 0.5 | 93.26 ± 0.3 | 86.24 ± 0.9 | −0.53 |
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Mare, R.; Da, H.; Fresta, M.; Cosco, D.; Awasthi, V. Anchoring Property of a Novel Hydrophilic Lipopolymer, HDAS-SHP, Post-Inserted in Preformed Liposomes. Nanomaterials 2019, 9, 1185. https://doi.org/10.3390/nano9091185
Mare R, Da H, Fresta M, Cosco D, Awasthi V. Anchoring Property of a Novel Hydrophilic Lipopolymer, HDAS-SHP, Post-Inserted in Preformed Liposomes. Nanomaterials. 2019; 9(9):1185. https://doi.org/10.3390/nano9091185
Chicago/Turabian StyleMare, Rosario, Huining Da, Massimo Fresta, Donato Cosco, and Vibhudutta Awasthi. 2019. "Anchoring Property of a Novel Hydrophilic Lipopolymer, HDAS-SHP, Post-Inserted in Preformed Liposomes" Nanomaterials 9, no. 9: 1185. https://doi.org/10.3390/nano9091185