Influence of Environmental Conditions on the Fusion of Cationic Liposomes with Living Mammalian Cells
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Description: Figure S1. Fluorescence and phase contrast micrographs of CHO cells upon treatment with fusogenic (FLs) (DOPE/DOTAP/TFPE-head 1/1/0.1 mol/mol) and endocytic (ELs) (DOPC/DOTAP/TFPE-head 1/1/0.1 mol/mol) liposomes. Cellular intercalation of the liposomes was verified by an additional washing step with a heparine solution. Uptake efficiencies are shown in the micrographs (%). Green: TFPE-head signal, red: nucleic staining with DRAQ5. Scale bar, 50 µm. Table S1. Temperature dependence of the fusion efficiencies of liposomes containing the cationic lipid DOTAP, different helper lipids, and TFPE-chain or DiR as an aromatic molecule (1/1/0.1 mol/mol). Efficiencies of endocytosis and fusion give a sum of 100%. Average values of at least three independent measurements and their standard deviations are given. Table S2. Fusion efficiencies of liposomes containing the cationic lipid DOTAP, different helper lipids, and TFPE-chain or DiR as dye molecule (1/1/0.1 mol/mol) depending on the osmolarity and ionic strength of the buffer. Efficiencies of endocytosis and fusion give a sum of 100%. Average values of at least three independent measurements and their standard deviations are given.
Kolašinac, R.; Jaksch, S.; Dreissen, G.; Braeutigam, A.; Merkel, R.; Csiszár, A. Influence of Environmental Conditions on the Fusion of Cationic Liposomes with Living Mammalian Cells. Nanomaterials 2019, 9, 1025. https://doi.org/10.3390/nano9071025
Kolašinac R, Jaksch S, Dreissen G, Braeutigam A, Merkel R, Csiszár A. Influence of Environmental Conditions on the Fusion of Cationic Liposomes with Living Mammalian Cells. Nanomaterials. 2019; 9(7):1025. https://doi.org/10.3390/nano9071025
Chicago/Turabian StyleKolašinac, Rejhana; Jaksch, Sebastian; Dreissen, Georg; Braeutigam, Andrea; Merkel, Rudolf; Csiszár, Agnes. 2019. "Influence of Environmental Conditions on the Fusion of Cationic Liposomes with Living Mammalian Cells" Nanomaterials 9, no. 7: 1025. https://doi.org/10.3390/nano9071025