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Nanomaterials 2018, 8(5), 278; https://doi.org/10.3390/nano8050278

Current Conjugation Methods for Immunosensors

1
and
2,3,*
1
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
2
Institute of Biomedical Engineering, College of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan
3
Department of Biological Science and Technology, College of Biological Science and Technology, National Chiao Tung University, Hsinchu 30010, Taiwan
*
Author to whom correspondence should be addressed.
Received: 3 April 2018 / Revised: 20 April 2018 / Accepted: 23 April 2018 / Published: 26 April 2018
(This article belongs to the Special Issue Nanomaterials with Functional Polymer Elements)
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Abstract

Recent advances in the development of immunosensors using polymeric nanomaterials and nanoparticles have enabled a wide range of new functions and applications in diagnostic and prognostic research. One fundamental challenge that all immunosensors must overcome is to provide the specificity of target molecular recognition by immobilizing antibodies, antibody fragments, and/or other peptides or oligonucleotide molecules that are capable of antigen recognition on a compact device surface. This review presents progress in the application of immobilization strategies including the classical adsorption process, affinity attachment, random cross-linking and specific covalent linking. The choice of immobilization methods and its impact on biosensor performance in terms of capture molecule loading, orientation, stability and capture efficiency are also discussed in this review. View Full-Text
Keywords: immunosensors; polymeric nanomaterials; immobilization methods immunosensors; polymeric nanomaterials; immobilization methods
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Li, Z.; Chen, G.-Y. Current Conjugation Methods for Immunosensors. Nanomaterials 2018, 8, 278.

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