Next Article in Journal
Dynamic Response of Graphitic Flakes in Nematic Liquid Crystals: Confinement and Host Effect
Next Article in Special Issue
Hollow Au–Ag Alloy Nanorices and Their Optical Properties
Previous Article in Journal
Thermal Conductivity of a Nanoscale Yttrium Iron Garnet Thin-Film Prepared by the Sol-Gel Process
Previous Article in Special Issue
Charge Transfer Effect on Raman and Surface Enhanced Raman Spectroscopy of Furfural Molecules
Open AccessArticle

Characterization and Discrimination of Gram-Positive Bacteria Using Raman Spectroscopy with the Aid of Principal Component Analysis

1
National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat, 400293 Cluj-Napoca, Romania
2
Faculty of Physics, Babeş-Bolyai University, 1 Kogălniceanu, 400084 Cluj-Napoca, Romania
3
Department of Food Science, University of Agricultural Science and Veterinary Medicine, 3-5 Calea Mănăştur, 400372 Cluj-Napoca, Romania
4
Faculty of Biology and Geology, Babeș-Bolyai University, 44 Republicii, 400015 Cluj-Napoca, Romania
*
Author to whom correspondence should be addressed.
Nanomaterials 2017, 7(9), 248; https://doi.org/10.3390/nano7090248
Received: 14 July 2017 / Revised: 27 August 2017 / Accepted: 28 August 2017 / Published: 1 September 2017
(This article belongs to the Special Issue Nanomaterials for SERS Applications)
Raman scattering and its particular effect, surface-enhanced Raman scattering (SERS), are whole-organism fingerprinting spectroscopic techniques that gain more and more popularity in bacterial detection. In this work, two relevant Gram-positive bacteria species, Lactobacillus casei (L. casei) and Listeria monocytogenes (L. monocytogenes) were characterized based on their Raman and SERS spectral fingerprints. The SERS spectra were used to identify the biochemical structures of the bacterial cell wall. Two synthesis methods of the SERS-active nanomaterials were used and the recorded spectra were analyzed. L. casei and L. monocytogenes were successfully discriminated by applying Principal Component Analysis (PCA) to their specific spectral data. View Full-Text
Keywords: Gram-positive bacteria; L. casei; L. monocytogenes; Raman scattering; SERS; silver nanoparticles Gram-positive bacteria; L. casei; L. monocytogenes; Raman scattering; SERS; silver nanoparticles
Show Figures

Graphical abstract

MDPI and ACS Style

Colniță, A.; Dina, N.E.; Leopold, N.; Vodnar, D.C.; Bogdan, D.; Porav, S.A.; David, L. Characterization and Discrimination of Gram-Positive Bacteria Using Raman Spectroscopy with the Aid of Principal Component Analysis. Nanomaterials 2017, 7, 248.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop