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Nanomaterials 2017, 7(4), 91;

The Interactions between ZnO Nanoparticles (NPs) and α-Linolenic Acid (LNA) Complexed to BSA Did Not Influence the Toxicity of ZnO NPs on HepG2 Cells

1,* and 1,2,*
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China
Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry Education, Laboratory of Biochemistry, College of Chemistry, Xiangtan University, Xiangtan 411105, China
These two authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Academic Editor: Thomas Nann
Received: 14 March 2017 / Revised: 10 April 2017 / Accepted: 20 April 2017 / Published: 24 April 2017
Full-Text   |   PDF [9663 KB, uploaded 24 April 2017]   |  


Background: Nanoparticles (NPs) entering the biological environment could interact with biomolecules, but little is known about the interaction between unsaturated fatty acids (UFA) and NPs. Methods: This study used α-linolenic acid (LNA) complexed to bovine serum albumin (BSA) for UFA and HepG2 cells for hepatocytes. The interactions between BSA or LNA and ZnO NPs were studied. Results: The presence of BSA or LNA affected the hydrodynamic size, zeta potential, UV-Vis, fluorescence, and synchronous fluorescence spectra of ZnO NPs, which indicated an interaction between BSA or LNA and NPs. Exposure to ZnO NPs with the presence of BSA significantly induced the damage to mitochondria and lysosomes in HepG2 cells, associated with an increase of intracellular Zn ions, but not intracellular superoxide. Paradoxically, the release of inflammatory cytokine interleukin-6 (IL-6) was decreased, which indicated the anti-inflammatory effects of ZnO NPs when BSA was present. The presence of LNA did not significantly affect all of these endpoints in HepG2 cells exposed to ZnO NPs and BSA. Conclusions: the results from the present study indicated that BSA-complexed LNA might modestly interact with ZnO NPs, but did not significantly affect ZnO NPs and BSA-induced biological effects in HepG2 cells. View Full-Text
Keywords: HepG2 cells; α-linolenic acid (LNA); ZnO nanoparticles; interaction HepG2 cells; α-linolenic acid (LNA); ZnO nanoparticles; interaction

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Zhou, Y.; Fang, X.; Gong, Y.; Xiao, A.; Xie, Y.; Liu, L.; Cao, Y. The Interactions between ZnO Nanoparticles (NPs) and α-Linolenic Acid (LNA) Complexed to BSA Did Not Influence the Toxicity of ZnO NPs on HepG2 Cells. Nanomaterials 2017, 7, 91.

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