Evaluation of Different Single-Walled Carbon Nanotube Surface Coatings for Single-Particle Tracking Applications in Biological Environments
Abstract
:1. Introduction
2. Results and Discussion
2.1. Cytotoxicity Experiments
2.2. Photoluminescence Imaging of Biocompatible Nanotubes
2.2.1. Photoluminescence Imaging of Biocompatible Nanotubes in Biological Medium
2.2.2. Photoluminescence Imaging of Biocompatible PLPEG- or F108-Coated SWCNT in Thick Biocompatible Aqueous Gels
3. Materials and Methods
3.1. Preparation of SWCNT Dispersions
3.2. Cell Culture Studies
3.3. Single Nanotube Fluorescence Microscopy Setup
3.4. Agarose Sample Preparation
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Nanotubes | Surfactant | Biological System | Dose | Exposure Time | Assay Method | Conclusion | Reference |
---|---|---|---|---|---|---|---|
HiPco SWCNTs | PLPEG | Human serum and intravenous injection in rats | 60 μg/mL | 0.5 h | ELISA | Activation of the complement system by SWCNTs in undiluted normal human serum and in vivo rats. | [18] |
HiPco SWCNTs | PLPEG | Intravenous/brain injection in rats | 60 μg/mL | 0.5 h to days | Fluorescence | In vivo SWCNT circulation (vascular system, brain). Stable imaging in vivo and in tissues. | [11,17] |
HiPco SWCNTs | Pluronic F108 | J774.1A mouse peritoneal macrophage | 11 ng/mL | 0, 8, 18 and 24 h | Fluorescence | Macrophages can ingest significant quantities of SWCNTs without showing toxic effects. Stable imaging in cells. | [19] |
HiPco SWCNTs | Pluoronic F127 | HeLa cells | 200 μg/mL | 2 days | Fluorescence imaging | Induction of actin bundling in cells, reduced cellular proliferation. | [20] |
Arc Discharge SWCNTs | Tween20 | Pathgen free guinea pigs | 50 mg/mL | 4 weeks | Lung function, bronchoalveolart lavega | No abnormalities of pulmonary function or measurable inflammation in guinea pigs. | [21] |
MWCNTs | Sterile saline + Brij 35 | Incubation with cytochrome P450 enzymes (CYP3A4) | 0.067 mg/mL | 5 min at 37 °C | Capillary electrochromatography, enzyme activity monitoring | No effect on CYP3A4 activity. Substantial improvement of migration time and peak shape repeatability in capillary electrochromatography. | [22] |
HiPco SWCNTs | ISPVP | Human embryonic kidney cells (HEK cells) | 1/30 μg/mL | 5 min at RT or 12 h at 37 °C | Fluorescence | Stable imaging in cells. | [23] |
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Gao, Z.; Danné, N.; Godin, A.G.; Lounis, B.; Cognet, L. Evaluation of Different Single-Walled Carbon Nanotube Surface Coatings for Single-Particle Tracking Applications in Biological Environments. Nanomaterials 2017, 7, 393. https://doi.org/10.3390/nano7110393
Gao Z, Danné N, Godin AG, Lounis B, Cognet L. Evaluation of Different Single-Walled Carbon Nanotube Surface Coatings for Single-Particle Tracking Applications in Biological Environments. Nanomaterials. 2017; 7(11):393. https://doi.org/10.3390/nano7110393
Chicago/Turabian StyleGao, Zhenghong, Noémie Danné, Antoine Guillaume Godin, Brahim Lounis, and Laurent Cognet. 2017. "Evaluation of Different Single-Walled Carbon Nanotube Surface Coatings for Single-Particle Tracking Applications in Biological Environments" Nanomaterials 7, no. 11: 393. https://doi.org/10.3390/nano7110393