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Article

Nanoparticle-Based Delivery of Anaplasma marginale Membrane Proteins; VirB9-1 and VirB10 Produced in the Pichia pastoris Expression System

1
Department of Agriculture and Fisheries, Agri-Science Queensland, Animal Science, St Lucia, QLD 4072, Australia
2
Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Australia
3
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia
4
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Neil O’Brien-Simpson
Nanomaterials 2016, 6(11), 201; https://doi.org/10.3390/nano6110201
Received: 12 August 2016 / Revised: 21 October 2016 / Accepted: 28 October 2016 / Published: 5 November 2016
(This article belongs to the Special Issue Nanoparticles in Immunology)
Bovine anaplasmosis or cattle-tick fever is a tick-borne haemolytic disease caused by the rickettsial haemoparasite Anaplasma marginale in tropical and subtropical areas of the world. While difficult to express, the proteins VirB9-1 and VirB10 are immunogenic components of the outer membrane type IV secretion system that have been identified as candidate antigens for vaccines targeting of A. marginale. Soluble VirB9-1 and VirB10 were successfully expressed using Pichia pastoris. When formulated with the self-adjuvanting silica vesicles, SV-100 (diameter: 50 nm, and pore entrance size: 6 nm), 200 µg of VirB9-1 and VirB10 were adsorbed per milligram of nanoparticle. The VirB9-1 and VirB10, SV-100 formulations were shown to induce higher antibody responses in mice compared to the QuilA formulations. Moreover, intracellular staining of selected cytokines demonstrated that both VirB9-1 and VirB10 formulations induced cell-mediated immune responses in mice. Importantly, the SV-100 VirB9-1 and VirB10 complexes were shown to specifically stimulate bovine T-cell linages derived from calves immunised with A. marginale outer membrane fractions, suggesting formulations will be useful for bovine immunisation and protection studies. Overall this study demonstrates the potential of self-adjuvanting silica vesicle formulations to address current deficiencies in vaccine delivery applications. View Full-Text
Keywords: Anaplasma marginale; VirB9-1; VirB10; Pichia pastoris; silica vesicles; vaccine adjuvants; immune responses Anaplasma marginale; VirB9-1; VirB10; Pichia pastoris; silica vesicles; vaccine adjuvants; immune responses
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MDPI and ACS Style

Zhang, B.; Cavallaro, A.S.; Mody, K.T.; Zhang, J.; Deringer, J.R.; Brown, W.C.; Mahony, T.J.; Yu, C.; Mitter, N. Nanoparticle-Based Delivery of Anaplasma marginale Membrane Proteins; VirB9-1 and VirB10 Produced in the Pichia pastoris Expression System. Nanomaterials 2016, 6, 201. https://doi.org/10.3390/nano6110201

AMA Style

Zhang B, Cavallaro AS, Mody KT, Zhang J, Deringer JR, Brown WC, Mahony TJ, Yu C, Mitter N. Nanoparticle-Based Delivery of Anaplasma marginale Membrane Proteins; VirB9-1 and VirB10 Produced in the Pichia pastoris Expression System. Nanomaterials. 2016; 6(11):201. https://doi.org/10.3390/nano6110201

Chicago/Turabian Style

Zhang, Bing; Cavallaro, Antonio S.; Mody, Karishma T.; Zhang, Jun; Deringer, James R.; Brown, Wendy C.; Mahony, Timothy J.; Yu, Chengzhong; Mitter, Neena. 2016. "Nanoparticle-Based Delivery of Anaplasma marginale Membrane Proteins; VirB9-1 and VirB10 Produced in the Pichia pastoris Expression System" Nanomaterials 6, no. 11: 201. https://doi.org/10.3390/nano6110201

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