Cytotoxicity of Silver Nanoparticle and Chitin-Nanofiber Sheet Composites Caused by Oxidative Stress
Abstract
:1. Introduction
2. Results
2.1. Cell Proliferation Assay and NO/NO2 Measurement In Vitro
2.2. Wound Closure and Histochemical Analyses
2.3. Carbonyl Protein
3. Discussion
4. Materials and Methods
4.1. Preparation of Ag NPs and Ag NPs/CNFS
4.2. Cell Proliferation Assay and NO/NO2 Measurement In Vitro
4.3. In Vivo Study
4.3.1. Wound Closure Analyses
4.3.2. Histochemistry
4.3.3. Measurements of Carbonyl Protein
4.4. Statistical Analyses
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
Ag NPs/CNFS | silver nanoparticles/chitin-nanofiber sheet composites |
8-OHdG | 8-hydroxy-2′-deoxyguanosine |
NO/NO2 | nitric oxide/nitrogen dioxide |
FBS | heat-inactivated fetal bovine serum |
DMEM | Dulbecco’s modified Eagle’s medium |
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Kinoda, J.; Ishihara, M.; Hattori, H.; Nakamura, S.; Fukuda, K.; Yokoe, H. Cytotoxicity of Silver Nanoparticle and Chitin-Nanofiber Sheet Composites Caused by Oxidative Stress. Nanomaterials 2016, 6, 189. https://doi.org/10.3390/nano6100189
Kinoda J, Ishihara M, Hattori H, Nakamura S, Fukuda K, Yokoe H. Cytotoxicity of Silver Nanoparticle and Chitin-Nanofiber Sheet Composites Caused by Oxidative Stress. Nanomaterials. 2016; 6(10):189. https://doi.org/10.3390/nano6100189
Chicago/Turabian StyleKinoda, Jun, Masayuki Ishihara, Hidemi Hattori, Shingo Nakamura, Koichi Fukuda, and Hidetaka Yokoe. 2016. "Cytotoxicity of Silver Nanoparticle and Chitin-Nanofiber Sheet Composites Caused by Oxidative Stress" Nanomaterials 6, no. 10: 189. https://doi.org/10.3390/nano6100189