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Open AccessArticle

Surface Stabilization Affects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells

1
Department of Clinical Laboratory Diagnostics, University Hospital Osijek, Josipa Huttlera 4, 31000 Osijek, Croatia
2
Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Josipa Huttlera 4, 31000 Osijek, Croatia
3
Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(7), 1390; https://doi.org/10.3390/nano10071390
Received: 19 June 2020 / Revised: 13 July 2020 / Accepted: 14 July 2020 / Published: 17 July 2020
(This article belongs to the Special Issue Safety and Biocompatibility of Metallic Nanoparticles)
Silver nanoparticles (AgNPs) are one of the most investigated metal-based nanomaterials. Their biocidal activity boosted their application in both diagnostic and therapeutic medical systems. It is therefore crucial to provide sound evidences for human-related safety of AgNPs. This study aimed to enhance scientific knowledge with regard to biomedical safety of AgNPs by investigating how their different surface properties affect human immune system. Methods: preparation, characterization and stability evaluation was performed for four differently coated AgNPs encompassing neutral, positive and negative agents used for their surface stabilization. Safety aspects were evaluated by testing interaction of AgNPs with fresh human peripheral blood mononuclear cells (hPBMC) by means of particle cellular uptake and their ability to trigger cell death, apoptosis and DNA damages through induction of oxidative stress and damages of mitochondrial membrane. Results: all tested AgNPs altered morphology of freshly isolated hPBMC inducing apoptosis and cell death in a dose- and time-dependent manner. Highest toxicity was observed for positively-charged and protein-coated AgNPs. Cellular uptake of AgNPs was also dose-dependently increased and highest for positively charged AgNPs. Intracellularly, AgNPs induced production of reactive oxygen species (ROS) and damaged mitochondrial membrane. Depending on the dose, all AgNPs exhibited genotoxic potential. Conclusions: this study provides systematic and comprehensive data showing how differently functionalized AgNPs may affect the human immune system. Presented results are a valuable scientific contribution to safety assessment of nanosilver-based blood-contacting medical products. View Full-Text
Keywords: nanosafety; surface coating; cellular uptake; apoptosis; oxidative stress; genotoxicity nanosafety; surface coating; cellular uptake; apoptosis; oxidative stress; genotoxicity
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MDPI and ACS Style

Vuković, B.; Milić, M.; Dobrošević, B.; Milić, M.; Ilić, K.; Pavičić, I.; Šerić, V.; Vrček, I.V. Surface Stabilization Affects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells. Nanomaterials 2020, 10, 1390. https://doi.org/10.3390/nano10071390

AMA Style

Vuković B, Milić M, Dobrošević B, Milić M, Ilić K, Pavičić I, Šerić V, Vrček IV. Surface Stabilization Affects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells. Nanomaterials. 2020; 10(7):1390. https://doi.org/10.3390/nano10071390

Chicago/Turabian Style

Vuković, Barbara; Milić, Marija; Dobrošević, Blaženka; Milić, Mirta; Ilić, Krunoslav; Pavičić, Ivan; Šerić, Vatroslav; Vrček, Ivana V. 2020. "Surface Stabilization Affects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells" Nanomaterials 10, no. 7: 1390. https://doi.org/10.3390/nano10071390

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