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Article

Ipriflavone-Loaded Mesoporous Nanospheres with Potential Applications for Periodontal Treatment

1
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 28040 Madrid, Spain
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Departamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, Plaza Ramón y Cajal s/n, 28040 Madrid, Spain
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CIBER de Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN, 28040 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Nanomaterials 2020, 10(12), 2573; https://doi.org/10.3390/nano10122573
Received: 3 December 2020 / Revised: 15 December 2020 / Accepted: 18 December 2020 / Published: 21 December 2020
(This article belongs to the Special Issue Nano-Engineering Solutions for Dental Implant Applications)
The incorporation and effects of hollow mesoporous nanospheres in the system SiO2–CaO (nanoMBGs) containing ipriflavone (IP), a synthetic isoflavone that prevents osteoporosis, were evaluated. Due to their superior porosity and capability to host drugs, these nanoparticles are designed as a potential alternative to conventional bioactive glasses for the treatment of periodontal defects. To identify the endocytic mechanisms by which these nanospheres are incorporated within the MC3T3-E1 cells, five inhibitors (cytochalasin B, cytochalasin D, chlorpromazine, genistein and wortmannin) were used before the addition of these nanoparticles labeled with fluorescein isothiocyanate (FITC–nanoMBGs). The results indicate that nanoMBGs enter the pre-osteoblasts mainly through clathrin-dependent mechanisms and in a lower proportion by macropinocytosis. The present study evidences the active incorporation of nanoMBG–IPs by MC3T3-E1 osteoprogenitor cells that stimulate their differentiation into mature osteoblast phenotype with increased alkaline phosphatase activity. The final aim of this study is to demonstrate the biocompatibility and osteogenic behavior of IP-loaded bioactive nanoparticles to be used for periodontal augmentation purposes and to shed light on internalization mechanisms that determine the incorporation of these nanoparticles into the cells. View Full-Text
Keywords: endocytosis; ipriflavone; mesoporous nanospheres; nanoparticles; oxidative stress; pre-osteoblasts endocytosis; ipriflavone; mesoporous nanospheres; nanoparticles; oxidative stress; pre-osteoblasts
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MDPI and ACS Style

Casarrubios, L.; Gómez-Cerezo, N.; Feito, M.J.; Vallet-Regí, M.; Arcos, D.; Portolés, M.T. Ipriflavone-Loaded Mesoporous Nanospheres with Potential Applications for Periodontal Treatment. Nanomaterials 2020, 10, 2573. https://doi.org/10.3390/nano10122573

AMA Style

Casarrubios L, Gómez-Cerezo N, Feito MJ, Vallet-Regí M, Arcos D, Portolés MT. Ipriflavone-Loaded Mesoporous Nanospheres with Potential Applications for Periodontal Treatment. Nanomaterials. 2020; 10(12):2573. https://doi.org/10.3390/nano10122573

Chicago/Turabian Style

Casarrubios, Laura, Natividad Gómez-Cerezo, María J. Feito, María Vallet-Regí, Daniel Arcos, and María T. Portolés 2020. "Ipriflavone-Loaded Mesoporous Nanospheres with Potential Applications for Periodontal Treatment" Nanomaterials 10, no. 12: 2573. https://doi.org/10.3390/nano10122573

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