Tetrodotoxin Retention in the Toxic Goby Yongeichthys criniger
Round 1
Reviewer 1 Report
This manuscript has innovative features and it complements previous studies conducted by other researchers. The experiment was planned correctly. Tables and charts are legible. I have no comments, my assessment of this paper is high and I recommend it for publication in the Journal of Marine Science and Engineering.
Author Response
Thank you very much for taking time out of your busy schedule to do the peer review.
Reviewer 2 Report
The manuscript describes a study of TTX absorption, transportation accumulation and retention in wild yongeichthys criniger specimens subjected to rearing experiments and fed a non-toxic diet. The reduction of the initial contamination of collected fish was determined at different experiment time and distribution, elimination rate in skin, muscle, liver and gonads were studied. The results showed a behavior for y. criniger different from that observed in some TTX-bearing pufferfish species. The paper is sufficiently well written even if English should be revised by a native English speaker. The results are clear and constitute an important contribution to the knowledge of TTX retention mechanism in fish. I think this paper will be suitable for publication in JMSE after the authors have addressed some minor issues.
Lines 43-44 – Can the author better explain this sentence?
Line 62 – Maybe is better to change the sentence in “The body weights and lengths of fish of Group I and Group II are shown in Tables 1 and 2, respectively”.
Line 116 – How much tissue homogenate does the author use for the analysis? And what is the volume of the extraction solvent added?
Line 117 – To what temperature are heated the samples? (boiling water??)
Line 134 – What means “as for Group I”?
Line 136 – Could the author give more information about the TTX quantification method. Did he use solvent standards or matrix matched standards? Wath was the calibration range?
Line 160 – How did the author calculate the TTX amount per individual in Group II fish? All of them were dissected.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf