Low Soil Phosphorus Availability Increases Acid Phosphatases Activities and Affects P Partitioning in Nodules, Seeds and Rhizosphere of Phaseolus vulgaris
Abstract
:1. Introduction
2. Results
2.1. Growth and Nodulation
2.2. Soil Available P
2.3. Acid Phosphatase and Phytase Activities in Rhizospheric Soils
2.4. Acid Phosphatase and Phytase Activities in Nodules and Seeds
2.5. Phosphorus and Phytate Distribution in Nodules and Seeds
Genotypes | Nodule P (mg g−1) | Seed P (mg g−1) | Nodule Phy (mg g−1) | Seed Phy (mg g−1) | ||||
S1 | S2 | S1 | S2 | S1 | S2 | S1 | S2 | |
L115 | 4.95 ± 0.5e | 7.8 ± 0.9a–d | 9.5 ± 0.64a | 5.9 ± 0.25ef | 4.8 ± 0.1cd | 5.9 ± 0.6bc | 6.2 ± 0.2ab | 4.9 ± 0.1c–f |
Fl | 9.3 ± 0.1a–c | 5.96 ± 1.3de | 6.2 ± 0.3d–f | 6.3 ± 0.3d–f | 6.3 ± 0.16b | 4.42 ± 0.3d | 5.7 ± 0.3a–d | 4.7 ± 0.2ef |
L147 | 9.6 ± 0.3ab | 7.8 ± 0.2a–d | 8.2 ± 0.6b | 7.24 ± 0.3ab | 6 ± 0.6bc | 5.9 ± 0.3bc | 5.8 ± 0.2a–c | 4.9 ± 0.2c–f |
Ct | 10.45 ± 0.55a | 9.2 ± 0.27a–c | 7.1 ± 0.2cd | 7.1 ± 0.3cd | 9.3 ± 0.4a | 6.4 ± 0.7b | 6.2 ± 0.3ab | 4.5 ± 0.3ef |
Br | 6.23 ± 1.4c–e | 5.91 ± 0.53de | 7.1 ± 0.2c–e | 5.51 ± 0.4f | 5.7 ± 0.2b–d | 4.8 ± 0.3cd | 6.6 ± 0.3a | 5.4 ± 0.3b–e |
Cs | 6.4 ± 1.1b–e | 5.96 ± 1.3de | 5.96 ± 0.2ef | 5.35 ± 0.3f | 6 ± 0.3bc | 5.4 ± 0.3b–d | 4.2 ± 0.1f | 4.9 ± 0.3d–f |
2.6. P Uptake, Use Efficiency and Absorption Efficiency by Root
2.7. Relationship between Nodulation, Shoot Growth, P and N Contents
3. Discussion
4. Experimental Section
4.1. Plant Material and Field Conditions
4.2. Soils Analyses
Characteristics | S1 | S2 |
---|---|---|
Clay (%) | 18.93 | 17.32 |
Sand (%) | 49.86 | 66.22 |
Silt (%) | 33.75 | 15.75 |
pH | 8.2 | 8.09 |
Organic matter (%) | 1.61 | 1.39 |
CaCO3 total (%) | 15 | 16.3 |
CaCO3 active (%) | 21 | 33 |
Ptotal (g Kg−1) | 1.6 | 0.54 |
Polsen (g Kg−1) | 0.0167 | 0.0043 |
Nitrogen (g Kg−1) | 1.21 | 1.09 |
K+ (g Kg−1) | 0.29 | 0.149 |
4.3. Harvest and Measurement of Plant, Nodule and Yield Components
4.4. APase and Phytase Activities Assays in Rhizosphere Soils
4.5. APase and Phytase Activities Assays in Nodules and Seeds
4.6. Determination of P, N, Phytate and Statistical Analyses
5. Conclusions
Acknowledgements
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Bargaz, A.; Faghire, M.; Abdi, N.; Farissi, M.; Sifi, B.; Drevon, J.-J.; Cherkaoui Ikbal, M.; Ghoulam, C. Low Soil Phosphorus Availability Increases Acid Phosphatases Activities and Affects P Partitioning in Nodules, Seeds and Rhizosphere of Phaseolus vulgaris. Agriculture 2012, 2, 139-153. https://doi.org/10.3390/agriculture2020139
Bargaz A, Faghire M, Abdi N, Farissi M, Sifi B, Drevon J-J, Cherkaoui Ikbal M, Ghoulam C. Low Soil Phosphorus Availability Increases Acid Phosphatases Activities and Affects P Partitioning in Nodules, Seeds and Rhizosphere of Phaseolus vulgaris. Agriculture. 2012; 2(2):139-153. https://doi.org/10.3390/agriculture2020139
Chicago/Turabian StyleBargaz, Adnane, Mustapha Faghire, Neila Abdi, Mohamed Farissi, Bouaziz Sifi, Jean-Jacques Drevon, Mohamed Cherkaoui Ikbal, and Cherki Ghoulam. 2012. "Low Soil Phosphorus Availability Increases Acid Phosphatases Activities and Affects P Partitioning in Nodules, Seeds and Rhizosphere of Phaseolus vulgaris" Agriculture 2, no. 2: 139-153. https://doi.org/10.3390/agriculture2020139