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Article

Using Simple Sequence Repeats in 9 Brassica Complex Species to Assess Hypertrophic Curd Induction

1
Food and Environment (Di3A), Department of Agriculture, University of Catania, 95131 Catania, Italy
2
Department of Medical Science, University of Turin, 10126 Turin, Italy
3
Department of Mathematical Science, Politecnico di Torino, 10126 Turin, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Rodomiro Ortiz
Agriculture 2021, 11(7), 622; https://doi.org/10.3390/agriculture11070622
Received: 31 March 2021 / Revised: 23 June 2021 / Accepted: 28 June 2021 / Published: 1 July 2021
(This article belongs to the Special Issue Breeding and Genetics of Horticultural Crops)
Five Simple Sequence Repeats (SSRs) were used to assess the relationship between inflorescence characteristics and their allelic variation in 53 Brassica oleracea and Brassica wild relatives (n = 9). Curd morphometric traits, such as weight (CW), height (CH), diameter (CD1), shape (CS) inflorescence curvature angle (CA), and its curd stem diameter (CD2), were measured. The aim of the work was to analyze the relationships among the allelic patterns of the SSRs primers utilized, and their status of homo or heterozygosity registered at each locus, as well as the inflorescence morphometric traits in order to individuate genomic regions stimulating the hypertrophy of this reproductive organ. The relationships found explain the diversity among B. oleracea complex species (n = 9) for the inflorescence size and structure, allowing important time reduction during the breeding process by crossing wild species, transferring useful resistance, and organoleptic and nutraceutical traits. The five SSRs loci were BoABI1, BoAP1, BoPLD1, BoTHL1, and PBCGSSRBo39. According to the allelic variation ascertained, we evaluated the heterozygosity index (H) for each SSR above cited. The results showed a significant interaction between the H index of the BoPLD1 gene and the inflorescence characteristics, summarized by the First Principal Component (PC1) (p-value = 0.0244); we ascertained a negative correlation between the H index and inflorescence characteristics, namely CW, CH, CD1, CD2, CA. The homozygosity BoPLD1 alelles, indicated by the H index, affect the inflorescence characteristics and broccoli and cauliflower yields. View Full-Text
Keywords: Brassica complex species; MADH–box genes; SSRs assay; heterozygosity index; allelic variance; curd morphometric traits Brassica complex species; MADH–box genes; SSRs assay; heterozygosity index; allelic variance; curd morphometric traits
MDPI and ACS Style

Treccarichi, S.; Di Gaetano, C.; Di Stefano, F.; Gasparini, M.; Branca, F. Using Simple Sequence Repeats in 9 Brassica Complex Species to Assess Hypertrophic Curd Induction. Agriculture 2021, 11, 622. https://doi.org/10.3390/agriculture11070622

AMA Style

Treccarichi S, Di Gaetano C, Di Stefano F, Gasparini M, Branca F. Using Simple Sequence Repeats in 9 Brassica Complex Species to Assess Hypertrophic Curd Induction. Agriculture. 2021; 11(7):622. https://doi.org/10.3390/agriculture11070622

Chicago/Turabian Style

Treccarichi, Simone, Cornelia Di Gaetano, Fulvio Di Stefano, Mauro Gasparini, and Ferdinando Branca. 2021. "Using Simple Sequence Repeats in 9 Brassica Complex Species to Assess Hypertrophic Curd Induction" Agriculture 11, no. 7: 622. https://doi.org/10.3390/agriculture11070622

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