Expression Analysis of DgD14, DgBRC1 and DgLsL in the Process of Chrysanthemum Lateral Bud Formation

Round 1

Reviewer 1 Report

line 160: microscope type, manufacturer, place. The equipment used in the experiment should be described in the method section, not the results.

The caption under the table, e.g. 2, is unclear. "The different lower case letters among different treatment groups indicate significant differences followed by Duncan’s test with p < 0.05" It should be clearly explained: Lower case letters a, b, c, d mean ... in which treatment groups? what are the important differences?

Author Response

Dear reviewer:

Thank you very much for your comments concerning our manuscript entitled “Expression Analysis of DgD14, DgBRC1 and DgLsL in the Process of Chrysanthemum Lateral Bud Formation”. Those comments are valuable and helpful for us to revise and improve our paper. We have studied comments carefully and made corrections again in order to meet with approval. The main corrections in the paper and the responds to your comments are as followings:

Point 1: Line 160: microscope type, manufacturer and place. The equipment used in the experiment should be described in the method section, not the results.

Response 1: We are very sorry for our negligence of this problem. As reviewer suggested, we have re-written the type and manufacturer information of the microscope on line 129. It is produced by Shanghai Liguang Precise Instrument Co.,Ltd. in Shanghai, and its type is LGX-30A. Besides, in the revised paper, the equipment used in our experiment has been described in Materials and Methods instead of Results.

Point 2: The caption under the table, e.g. 2, is unclear. "The different lower case letters among different treatment groups indicate significant differences followed by Duncan’s test with p < 0.05" It should be clearly explained: Lower case letters a, b, c, d mean ... in which treatment groups? What are the important differences?

Response 2: It is really true as reviewer suggested. Therefore, we have made revisions in the captions under Table 2-3 and Figure 4-6. Lowercase letters a, b, c and d in Table 2-3 represent significant differences in lateral bud length after Duncan’s test (p < 0.05) between GA, ABA, IAA and control groups 0, 3 and 6 days after treatments.

Meanwhile, lowercase letters a, b and c in Figure 4-6 represent significant differences in expression levels of DgD14, DgBRC1 and DgLsL after Duncan’s test (p < 0.05) between GA, ABA, IAA and control groups after different time points treatments in ‘Jinba’ and ‘Fenyan’.

We tried our best to improve the manuscript again based on your comments. These changes will not influence the content and framework of the paper. Revised portions were marked in red in the paper. We appreciate for your warm work earnestly, and sincerely hope the correction will meet with approval.

Once again, thank you very much for your comments and suggestions!

Sincerely,

Cheng Luo, Xin-Jie Wang, Ai-Ning Ran, Jing-Jing Song, Xin Li, Qi-Qi Ma, Yuan-Zhi Pan, Qing-Lin Liu and Bei-Bei Jiang

13 November 2021

Reviewer 2 Report

Authors have addressed all the concerns. 

Author Response

Dear reviewer:

We would like to appreciate you a lot for your comments concerning our manuscript entitled “Expression Analysis of DgD14, DgBRC1 and DgLsL in the Process of Chrysanthemum Lateral Bud Formation”. Thank you very much for your recognition and affirmation of our manuscript. We tried our best to further revise and improve the whole paper again, making some changes in some respects. The revised portions were marked in red in the new manuscript. We appreciate for your warm work earnestly, and sincerely hope the corrections will meet with approval.

Once again, thank you very much for your comments and agreements!

Sincerely,

Cheng Luo, Xin-Jie Wang, Ai-Ning Ran, Jing-Jing Song, Xin Li, Qi-Qi Ma, Yuan-Zhi Pan, Qing-Lin Liu and Bei-Bei Jiang

13 November 2021

This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.

Round 1

Reviewer 1 Report

Authors have tried to correlate the gene regulation of DgD14, DgBRC1 and DgLsL with exogenous application of growth regulators  upon lateral bud formation. This current version of manuscript have several issues. Most critical is the novelty, and experimental procedure, it lacks the controls experiments. Thus need to improve this manuscript with new experimental design to perform proposed work. 

Reviewer 2 Report

Manuscript "Expression Analysis of DgD14, DgBRC1 and DgLsL in the Process of Chrysanthemum Lateral Bud Formation" describes the issue related to expression pattern of three genes in the process of lateral bud formation, and analyze the effects of different exogenous hormones on the growth of lateral bud and gene expression. 

The manuscript is prepared very carefully, methods are describe in great detail. The figures are legible and transparent. I can't find any stylistical and spelling errors. The citations are also correct. 

Author Response

Dear Reviewers:

We have carefully read your comments concerning our manuscript entitled “Expression Analysis of DgD14, DgBRC1 and DgLsL in the Process of Chrysanthemum Lateral Bud Formation”.Thank you very much for your recognition and affirmation of our manuscript! Next, we will try our best to further revise and improve our paper, making some changes in it. Hope that the correction will meet with approval.

Once again, thank you very much for your comments and agreements!

Reviewer 3 Report

I would like to give the following recommendations to authors:
Materials and Methods

Line 111 What company is the incubator, where was it produced?

Line 118 Instead of 50mg/L it should be 50 mg/L. It does not appear from the sentence whether the seedlings were sprayed with the mixture of IAA, GA, ABA and distilled water or with each substance separately.

Line 137 Incorrect spelling, two parentheses next to each other.

The methods lack information on how the pictures in Figure 2, 3 were taken, and with what approximation.

There is also no paragraph specifying the statistics that are used to compile the results.

How was the length of the side buds measured?

Results

In subsection 3.1. one by one is Table 1, 2 and Figure 2, 3 with no text, explanations in between.

The text under Figures 2 and 3 is difficult to read. What hormones were used? CK means the same as CKs?

"The different lower case letters among different treatment groups indicate significant differences followed by Duncan’s test with p < 0.05" What do the letters a, b, c mean in tables 1 and 2, Figure 4-6?

References

Please standardize the form of writing the quoted literature and update with new reports.